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1. Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression

Author:
Kihara, Takahiro; Noguchi, Takuya; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji
Source:
Bioresource technology reports 2019 v.7 pp. 100185
ISSN:
2589-014X
Subject:
xylose,   etc   ; Clostridium saccharoperbutylacetonicum; catabolite repression; cellobiose; continuous fermentation; glucose; recycling;     Show all 7 Subjects
Abstract:
... Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE) fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repressio ...
DOI:
10.1016/j.biteb.2019.03.017

2. Kinetic study of butanol production from mixtures of glucose and xylose and investigation of different pre-growth strategies

Author:
Birgen, Cansu; Berglihn, Olaf Trygve; Preisig, Heinz A.; Wentzel, Alexander
Source:
Biochemical engineering journal 2019 v.147 pp. 110-117
ISSN:
1369-703X
Subject:
xylose,   etc   ; biomass; butanol; dynamic models; fermentation; glucose; lignocellulose; microorganisms;     Show all 8 Subjects
Abstract:
... This study proposes a dynamic model that describes key characteristics of fermentative butanol production from glucose and xylose mixtures. The model has 12 parameters and incorporates noncompetitive inhibitory interaction between sugars as well as inhibitions due to high substrate and butanol concentrations. Different pre-growth strategies to achieve co-utilization of sugars were explored togethe ...
DOI:
10.1016/j.bej.2019.04.002

3. Pretreatment and enzymatic saccharification of oak at high solids loadings to obtain high titers and high yields of sugars

Author:
Kim, Dong Hyun; Park, Hyun Min; Jung, Young Hoon; Sukyai, Prakit; Kim, Kyoung Heon
Source:
Bioresource technology 2019 v.284 pp. 391-397
ISSN:
0960-8524
Subject:
xylose,   etc   ; biobased products; glucans; glucose; industry; lignocellulose; saccharification; slurries; xylan;     Show all 9 Subjects
Abstract:
... Production of high-titer sugar from lignocellulose is important in terms of process economics of bio-based product industry. In this study, to obtain high titers and yields of sugars, we combined pretreatment and saccharification steps, both at high solids loadings. First, pretreatment of oak was optimized at a 30% (w/w) solids loading. The whole slurry of the pretreated oak was subjected to a fed ...
DOI:
10.1016/j.biortech.2019.03.134

4. Synthesis of 2-fluoro-substituted and 2,6-modified purine 2′,3′-dideoxy-2′,3′-difluoro-d-arabinofuranosyl nucleosides from d-xylose

Author:
Sivets, Grigorii G.; Amblard, Franck; Schinazi, Raymond F.
Source:
Tetrahedron 2019 v.75 no.13 pp. 2037-2046
ISSN:
0040-4020
Subject:
xylose,   etc   ; chemical structure; glycosylation; nucleosides; organobromine compounds; salts;     Show all 6 Subjects
Abstract:
... A series of novel purine-modified 2′,3′-dideoxy-2′,3′-difluro-D-arabinonucleosides, including fluorinated analogs of fludarabine and nelarabine, have been prepared via anion glycosylation reactions of salts of 2-fluoropurine derivatives with the glycosyl bromide. A short and efficient synthetic route to the carbohydrate precursor 5-O-benzoyl-2,3-difluoro-α-d-arabinofuranosyl bromide was developed ...
DOI:
10.1016/j.tet.2019.02.027

5. Effective Mechanism of (−)-Epigallocatechin Gallate Indicating the Critical Formation Conditions of Amadori Compound during an Aqueous Maillard Reaction

Author:
Yu, Xiaohong; Cui, Heping; Hayat, Khizar; Hussain, Shahzad; Jia, Chengsheng; Zhang, Song-Lin; Tahir, Muhammad Usman; Zhang, Xiaoming; Ho, Chi-Tang
Source:
Journal of agricultural and food chemistry 2019 v.67 no.12 pp. 3412-3422
ISSN:
1520-5118
Subject:
xylose,   etc   ; Maillard reaction products; alanine; epigallocatechin gallate; temperature;     Show all 5 Subjects
Abstract:
... The formation conditions of the Amadori compound (ARP) N-(1-deoxy-d-xylulos-1-yl)-alanine were determined in an aqueous Maillard reaction between l-alanine and d-xylose under a two-step temperature rising process with (−)-epigallocatechin gallate (EGCG) as an indicator followed by browning intensity detection of the final Maillard reaction products (MRPs). To clarify the mechanism of EGCG indicati ...
DOI:
10.1021/acs.jafc.9b00034

6. Highly reactive 2-deoxy-2-iodo-d-allo and d-gulo pyranosyl sulfoxide donors ensure β-stereoselective glycosylations with steroidal aglycones

Author:
Mestre, Jordi; Collado, David; Benito-Alifonso, David; Rodríguez, Miguel A.; Matheu, M. Isabel; Díaz, Yolanda; Castillón, Sergio; Boutureira, Omar
Source:
RSC advances 2018 v.8 no.53 pp. 30076-30079
ISSN:
2046-2069
Subject:
xylose,   etc   ; glycosides; glycosylation; stereoselectivity; temperature;     Show all 5 Subjects
Abstract:
... The preparation of well-defined d-xylo and d-ribo glycosides represents a synthetic challenge due to the limited configurational availability of starting materials and the laborious synthesis of homogeneous 2-deoxy-β-glycosidic linkages, in particular that of the sugar-steroid motif, which represents the “stereoselective determining step” of the overall synthesis. Herein we describe the use of 2-d ...
DOI:
10.1039/c8ra06619a

7. Identification of a native promoter PLH-77 for gene expression in Paenibacillus polymyxa

Author:
Li, Hui; Ding, Yanqin; Zhao, Jianzhi; Ge, Ruofei; Qiu, Benhua; Yang, Xiaoli; Yao, Liangtong; Liu, Kai; Wang, Chengqiang; Du, Binghai
Source:
Journal of biotechnology 2019 v.295 pp. 19-27
ISSN:
0168-1656
Subject:
xylose; xylose isomerase,   etc   ; Paenibacillus polymyxa; Rhizobium; acetates; bacteria; fermentation; gene expression; genes; genetic improvement; green fluorescent protein; lactic acid; metabolites; monitoring; plant growth; screening;     Show all 16 Subjects
Abstract:
... Paenibacillus polymyxa is a rhizobacterium that has attracted substantial attention due to its ability to produce functional metabolites and promote plant growth. Metabolic and genetic improvements in this species will benefit research and other applications of the bacterium. However, a suitable gene expression system has not been established in this species. In this study, a promoter trap system ...
DOI:
10.1016/j.jbiotec.2019.02.002

8. Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan

Author:
Yamamoto, Yohei; Kishimura, Hideki; Kinoshita, Yasunori; Saburi, Wataru; Kumagai, Yuya; Yasui, Hajime; Ojima, Takao
Source:
Process biochemistry 2019 v.82 pp. 117-122
ISSN:
1359-5113
Subject:
xylose,   etc   ; Palmaria; cellulose; enzymes; hydrolysates; hydrolysis; lignin; pH; xylan; xylooligosaccharides; Japan;     Show all 11 Subjects
Abstract:
... Red alga dulse (Palmaria sp.) has xylan with a small amount of cellulose and less lignin, hence these characteristics may provide some advantages in the enzymatic production of xylooligosaccharides (XOS). To evaluate dulse xylan as XOS source, we prepared xylan rich fraction (DXRF) and attempted XOS production using commercial enzymes. 13.8% of xylan rich fraction (DXRF: containing 52.2% of xylan) ...
DOI:
10.1016/j.procbio.2019.03.030

9. Expansin assisted bio-affinity immobilization of endoxylanase from Bacillus subtilis onto corncob residue: Characterization and efficient production of xylooligosaccharides

Author:
Wu, Bin; Yu, Qi; Chang, Siyuan; Pedroso, Marcelo Monteiro; Gao, Zhen; He, Bingfang; Schenk, Gerhard
Source:
Food chemistry 2019 v.282 pp. 101-108
ISSN:
0308-8146
Subject:
xylose,   etc   ; Bacillus subtilis; adsorption; cellulose; corn cobs; endo-1,4-beta-xylanase; hydrolysis; xylan; xylooligosaccharides;     Show all 9 Subjects
Abstract:
... A one-step method to immobilize xylanase onto cellulosic material by fusion of expansin from Bacillus subtilis to xylanase LC9 without the requirement of prior purification of enzyme has been developed. Fusion enzyme EXLX-R2-XYN was specifically adsorbed onto corncob residue with high loading capacity due to bio-affinity adsorption of expansin onto cellulose. The immobilization yield was close to ...
DOI:
10.1016/j.foodchem.2019.01.004

10. Co-production of 1,2,4-butantriol and ethanol from lignocellulose hydrolysates

Author:
Zhao, Meilin; Shi, Dingchang; Lu, Xinyao; Zong, Hong; Zhuge, Bin
Source:
Bioresource technology 2019 v.282 pp. 433-438
ISSN:
0960-8524
Subject:
xylose,   etc   ; Candida; ethanol; fermentation; furfural; genes; glucose; hydrolysates; lignocellulose; osmolality; sugarcane bagasse; value-added products;     Show all 12 Subjects
Abstract:
... The aim of this work was to realize 1,2,4-butantriol (BT) production from sugarcane bagasse hydrolysates by microbial fermentation, and obtain co-production of BT and ethanol. Candida glycerinogenes UG21 was utilized to reduce the effect of osmolality resulting from high glucose concentration and furfural in hydrolysates on cell growth of BT-producing strains, and produced 54.1 g/L ethanol from gl ...
DOI:
10.1016/j.biortech.2019.03.057

11. Electrodialytic bioproduction of xylonic acid in a bioreactor of supplied-oxygen intensification by using immobilized whole-cell Gluconobacter oxydans as biocatalyst

Author:
Zhou, Xin; Han, Jian; Xu, Yong
Source:
Bioresource technology 2019 v.282 pp. 378-383
ISSN:
0960-8524
Subject:
xylose,   etc   ; Gluconobacter oxydans; aerobes; bacteria; biocatalysts; bioreactors; cost effectiveness; fermentation; immobilized cells; oxygen;     Show all 10 Subjects
Abstract:
... Immobilized whole-cell fermentation has been proven to be an effective method to improve the performance and cost-effectiveness of Gluconobacter oxydans ATCC 621. In the bio-oxidation of xylose to xylonic acid, the oxygen supply through the immobilized beads is a well-known factor that limits the biocatalytic performance of Gluconobacter oxydans as obligate aerobic bacteria. The activity of immobi ...
DOI:
10.1016/j.biortech.2019.03.042

12. Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery

Author:
Haavisto, Johanna M.; Lakaniemi, Aino-Maija; Puhakka, Jaakko A.
Source:
Environmental technology 2019 v.40 no.11 pp. 1467-1475
ISSN:
1479-487X
Subject:
xylose,   etc   ; energy; enrichment culture; freezing; microbial fuel cells; power generation; staining; storage temperature; volatile fatty acids;     Show all 9 Subjects
Abstract:
... Starting up a microbial fuel cell (MFC) requires often a long-term culture enrichment period, which is a challenge after process upsets. The purpose of this study was to develop low-cost storage for MFC enrichment culture to enable prompt process recovery after upsets. Anolyte of an operating xylose-fed MFC was stored at different temperatures and for different time periods. Storing the anolyte fo ...
DOI:
10.1080/09593330.2017.1423395

13. Ternary deep eutectic solvents for effective biomass deconstruction at high solids and low enzyme loadings

Author:
Chen, Zhu; Jacoby, William A.; Wan, Caixia
Source:
Bioresource technology 2019 v.279 pp. 281-286
ISSN:
0960-8524
Subject:
xylose,   etc   ; biomass; choline; ethylene glycol; glucose; guanidines; hydrogen bonding; hydrolysates; lignin; lignocellulose; propylene glycol; solvents; xylan;     Show all 13 Subjects
Abstract:
... Ternary deep eutectic solvents (DESs) were developed to enable rapid and high-solid biomass pretreatment as well as concentrated sugar hydrolysate production. Six ternary DESs constituted choline chloride (ChCl) or guanidine hydrochloride (GH) as a hydrogen bond acceptor (HBA), ethylene glycol (EG) or propylene glycol (PG) or glycerin (GLY) as a polyol-based hydrogen bond donor (HBD), and p-toluen ...
DOI:
10.1016/j.biortech.2019.01.126

14. Efficient Biosynthesis of Xylitol from Xylose by Coexpression of Xylose Reductase and Glucose Dehydrogenase in Escherichia coli

Author:
Jin, Li-Qun; Xu, Wei; Yang, Bo; Liu, Zhi-Qiang; Zheng, Yu-Guo
Source:
Applied biochemistry and biotechnology 2019 v.187 no.4 pp. 1143-1157
ISSN:
0273-2289
Subject:
xylose,   etc   ; Escherichia coli; Exiguobacterium; Rhizopus oryzae; biocatalysts; biosynthesis; biotransformation; enzyme activity; enzymes; glucose; industry; xylitol;     Show all 12 Subjects
Abstract:
... Xylitol is an important functional sugar alcohol which is widely used in industries. To efficiently produce xylitol from xylose by xylose reductase in a single step, the coupled system and the coexpression system using xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum were constructed, the latter of which was used for cofactor regeneration. O ...
DOI:
10.1007/s12010-018-2878-0

15. Ethanol production using Zymomonas mobilis: Development of a kinetic model describing glucose and xylose co-fermentation

Author:
Grisales Díaz, Víctor Hugo; Willis, Mark J.
Source:
Biomass and bioenergy 2019 v.123 pp. 41-50
ISSN:
0961-9534
Subject:
xylose,   etc   ; Zymomonas mobilis; biomass; ethanol; ethanol production; fermentation; glucose; kinetics; lignocellulose; prediction; xylitol;     Show all 11 Subjects
Abstract:
... Reliable kinetic models are important for the design and optimisation of lignocellulosic ethanol production. A new kinetic model describing ethanol production using Zymomonas mobilis ZM4(pZB5) that is applicable to single (glucose or xylose) or mixed (glucose and xylose) substrate fermentations is developed. This work extends previous contributions through consideration of an empirical term for th ...
DOI:
10.1016/j.biombioe.2019.02.004

16. Production of mannosylerythritol lipids from lignocellulose hydrolysates: tolerance thresholds of Moesziomyces antarcticus to inhibitors

Author:
Santos, Marisa V; Faria, Nuno T; Fonseca, César; Ferreira, Frederico Castelo
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.4 pp. 1064-1072
ISSN:
0268-2575
Subject:
xylose,   etc   ; acetates; biomass; biosurfactants; byproducts; equations; formates; furfural; glucose; hydrolysates; lignocellulose; lipids; model validation; models; yeasts;     Show all 15 Subjects
Abstract:
... BACKGROUND: Moesziomyces antarcticus is an efficient producer of mannosylerythritol lipids (MEL), a biosurfactant with a wide range of potential applications. The use of lignocellulosic biomass can contribute to sustainable MEL production. While lignocellulosic sugars (e.g. D‐glucose and D‐xylose) can be converted to MEL, the required pretreatment of lignocellulosic biomass releases by‐products th ...
DOI:
10.1002/jctb.5853

17. Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder

Author:
Gu, Bon-Jae; Dhumal, Gaurav S.; Wolcott, Michael P.; Ganjyal, Girish M.
Source:
Bioresource technology 2019 v.275 pp. 266-271
ISSN:
0960-8524
Subject:
xylose,   etc   ; biomass; cellulose; crystal structure; glucose; lignocellulose; mannose; particle size distribution; pretreatment; temperature; wood fibers;     Show all 11 Subjects
Abstract:
... Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars. Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield. Lower temperatures and screw speeds resulted in increased ...
DOI:
10.1016/j.biortech.2018.12.033

18. Asymmetric synthesis of the cytotoxic lactone (+)-cardiobutanolide and two novel analogues

Author:
Kovačević, Ivana; Popsavin, Mirjana; Rodić, Marko V.; Kojić, Vesna; Popsavin, Velimir
Source:
Tetrahedron letters 2019 v.60 no.9 pp. 684-687
ISSN:
0040-4039
Subject:
xylose,   etc   ; antineoplastic activity; chemical structure; cytotoxicity; dihydroxylation; doxorubicin; human cell lines; humans; neoplasm cells; stereoselectivity; zinc;     Show all 11 Subjects
Abstract:
... A new asymmetric synthesis of the naturally occurring styryl lactone cardiobutanolide and two novel analogues have been achieved starting from d-xylose. The key steps of the synthesis included an initial zinc mediated reductive THF-ring opening, stereoselective olefination and Sharpless asymmetric dihydroxylation. It was shown that cardiobutanolide (1) exhibits promising in vitro antitumour proper ...
DOI:
10.1016/j.tetlet.2019.01.059

19. High-yield production of D-1,2,4-butanetriol from lignocellulose-derived xylose by using a synthetic enzyme cascade in a cell-free system

Author:
Gao, Qian; Wang, Xin; Hu, Shewei; Xu, Nana; Jiang, Mingjun; Ma, Chen; Yang, Jianming; Xu, Sheng; Chen, Kequan; Ouyang, Pingkai
Source:
Journal of biotechnology 2019 v.292 pp. 76-83
ISSN:
0168-1656
Subject:
xylose,   etc   ; additives; biomass; buffers; cell free system; corn cobs; enzymes; fermentation; hydrolysates; metabolic engineering; pH; temperature;     Show all 12 Subjects
Abstract:
... Approaches using metabolic engineering to produce D-1, 2, 4-butanetriol (BT) from renewable biomass in microbial systems have achieved initial success. However, due to the lack of incomplete understanding of the complex branch pathway, the efficient fermentation system for BT production was difficult to develop. Here we reconstituted a cell-free system in vitro using purified enzymes to produce BT ...
DOI:
10.1016/j.jbiotec.2019.01.004

20. Fast screening of optimal acid-pretreatment conditions in the conversion of wood to lignocellulosic sugars

Author:
Smits, Jan; Bevers, Loes; van Haastert, Marco; Wiertz, Roel; Kroon, Hans
Source:
Bioresource technology reports 2019 v.5 pp. 220-229
ISSN:
2589-014X
Subject:
xylose,   etc   ; Picea; Populus; enzymatic hydrolysis; glucans; glucose; lignocellulose; mannose; screening; softwood;     Show all 10 Subjects
Abstract:
... A screening method is developed to identify the optimal acid pretreatment conditions in the production of cellulosic sugars from woods. The method is developed on gram scale and allows evaluation of more than 30 conditions within a week. Estimation of the residual glucan content after pretreatment is used to identify the optimal pretreatment conditions, being the maximal sugar release combined wit ...
DOI:
10.1016/j.biteb.2019.01.016