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1. Polyhydroxybutyrate production and increased macromolecule content in Chlamydomonas reinhardtii cultivated with xylose and reduced nitrogen levels

Author:
Cassuriaga, Ana Paula Aguiar; Moraes, Luiza; Morais, Michele Greque; Costa, Jorge Alberto Vieira
Source:
International journal of biological macromolecules 2020 v.158 pp. 875-883
ISSN:
0141-8130
Subject:
xylose,   etc   ; Chlamydomonas reinhardtii; ammonium chloride; nitrogen; pentoses; polyhydroxybutyrate; proteins;     Show all 7 Subjects
Abstract:
... The aim of the current study was to evaluate the production of macromolecules such as polyhydroxybutyrate under pentose supplementation and reduced nitrogen levels in Chlamydomonas reinhardtii. Two batches of experiments were carried out: (1) reduction in the nitrogen (NH₄Cl) concentration to 6 and 4 g L⁻¹ and (2) supplementation of 10, 20 and 30 mg L⁻¹ D-xylose together with a reduction in the NH ...
DOI:
10.1016/j.ijbiomac.2020.04.273

2. Mutarotation of aldoses: Getting a deeper knowledge of a classic equilibrium enabled by computational analyses

Author:
de la Concepción, Juan García; Martínez, R. Fernando; Cintas, Pedro; Babiano, Reyes
Source:
Carbohydrate research 2020 v.490 pp. 107964
ISSN:
0008-6215
Subject:
xylose,   etc   ; aldehydes; aldoses; energy; glucose; pH; solvents; temperature;     Show all 8 Subjects
Abstract:
... The mutarotation equilibrium, by which reducing carbohydrates exist in solution as the α and β anomers of cyclic (furanoid and pyranoid) structures, along with open-chain (aldehyde and hydrate) forms, and whose ratios are depending on factors such as temperature, pH and solvent, portraits a phenomenon involved in numerous processes of chemical and biological importance. Herein, we have developed a ...
DOI:
10.1016/j.carres.2020.107964

3. Recombinant xylose-fermenting yeast construction for the co-production of ethanol and cis,cis-muconic acid from lignocellulosic biomass

Author:
Liu, Tingting; Peng, Bingyin; Huang, Shuangcheng; Geng, Anli
Source:
Bioresource technology reports 2020 v.9 pp. 100395
ISSN:
2589-014X
Subject:
xylose; xylose isomerase,   etc   ; Bacteroides; Elaeis guineensis; Saccharomyces cerevisiae; aerobic conditions; biomass; catechol; ethanol; fermentation; fruits; gene overexpression; glucose; hydrolysates; lignocellulose; mutants; pentose phosphate cycle; stop codon; yeasts;     Show all 19 Subjects
Abstract:
... New exogenous cis,cis-muconic acid biosynthetic pathway genes were expressed in Saccharomyces cerevisiae. The xylose isomerase gene from Bacteroides valgutus and pentose phosphate pathway genes from S. cerevisiae were overexpressed in the yeast strain. The strain was further modified by the overexpression of gene Aro1 (with a stop codon of AroE) and a feedback-resistant Aro4opt mutant gene from S. ...
DOI:
10.1016/j.biteb.2020.100395

4. A differential evolution approach to estimate parameters in a temperature-dependent kinetic model for second generation ethanol production under high cell density with Spathaspora passalidarum

Author:
Biazi, L.E.; Martínez-Jimenez, F.D.; Bonan, C.I.D.G.; Soares, L.B.; Morais, E.R.; Ienczak, J.L.; Costa, A.C.
Source:
Biochemical engineering journal 2020 v.161 pp. 107586
ISSN:
1369-703X
Subject:
xylose,   etc   ; algorithms; cell growth; equations; ethanol fermentation; ethanol production; glucose; kinetics; simulation models; temperature; yeasts;     Show all 11 Subjects
Abstract:
... An unstructured–unsegregated temperature-dependent kinetic model was developed and validated to describe ethanol fermentation in a mixture of xylose and glucose for the nonconventional yeast Spathaspora passalidarum NRRL Y-27907 under high cell density in the temperature range of 26–32 °C. The kinetic model consisted of 13 equations and 16 kinetic parameters, describing cell growth, individual xyl ...
DOI:
10.1016/j.bej.2020.107586

5. Pitfalls in the 3, 5-dinitrosalicylic acid (DNS) assay for the reducing sugars: Interference of furfural and 5-hydroxymethylfurfural

Author:
Deshavath, Narendra Naik; Mukherjee, Gourab; Goud, Vaibhav V.; Veeranki, Venkata Dasu; Sastri, Chivukula V.
Source:
International journal of biological macromolecules 2020 v.156 pp. 180-185
ISSN:
0141-8130
Subject:
xylose,   etc   ; arabinose; biofuels; biomass; carbonyl compounds; furfural; glucose; hydroxymethylfurfural; lignocellulose; moieties; oxidation; reducing sugars; value added;     Show all 13 Subjects
Abstract:
... Transformation of renewable biomass into value-added chemicals and biofuels has evolved to be a vital field of research in recent years. Accurate estimation of reducing sugars post pretreatment of lignocellulosic biomass has been very inconsistent. For a few decades, 3,5-dinitrosalicylic acid (DNS) assay has been widely employed for the estimation of reducing sugars derived from pretreatment of li ...
DOI:
10.1016/j.ijbiomac.2020.04.045

6. Caproate production from xylose by mesophilic mixed culture fermentation

Author:
Qian, Ding-Kang; Geng, Zi-Qian; Sun, Ting; Dai, Kun; Zhang, Wei; Jianxiong Zeng, Raymond; Zhang, Fang
Source:
Bioresource technology 2020 v.308 pp. 123318
ISSN:
0960-8524
Subject:
xylose,   etc   ; batch systems; beta oxidation; biosynthesis; hexanoic acid; mixed culture; pH;     Show all 7 Subjects
Abstract:
... Caproate production by mixed culture fermentation (MCF) is economically attractive. Xylose is known as the second most abundant sugar in nature, however, producing caproate from xylose is never reported. In this study, caproate production from xylose by mesophilic MCF was firstly investigated. The results showed that as pH decreasing to 5.0, the caproate concentration was 2.06 g/L in a batch react ...
DOI:
10.1016/j.biortech.2020.123318

7. Dynamic consolidated bioprocessing for direct production of xylonate and shikimate from xylan by Escherichia coli

Author:
Gao, Cong; Guo, Liang; Ding, Qiang; Hu, Guipeng; Ye, Chao; Liu, Jia; Chen, Xiulai; Liu, Liming
Source:
Metabolic engineering 2020 v.60 pp. 128-137
ISSN:
1096-7176
Subject:
xylose,   etc   ; Escherichia coli; biomass production; bioprocessing; biosynthesis; carbon; feedstocks; value added; xylan; xylanases;     Show all 10 Subjects
Abstract:
... Numerous value-added chemicals can be produced using xylan as a feedstock. However, the product yields are limited by low xylan utilization efficiency, as well as by carbon flux competition between biomass production and biosynthesis. Herein, a dynamic consolidated bioprocessing strategy was developed, which coupled xylan utilization and yield optimization modules. Specifically, we achieved the ef ...
DOI:
10.1016/j.ymben.2020.04.001

8. Second-generation itaconic acid: An alternative product for biorefineries?

Author:
Magalhães, Antonio Irineudo; de Carvalho, Júlio Cesar; Thoms, Juliano Feliz; Souza Silva, Rafaeli; Soccol, Carlos Ricardo
Source:
Bioresource technology 2020 v.308 pp. 123319
ISSN:
0960-8524
Subject:
xylose,   etc   ; Aspergillus terreus; acetic acid; biorefining; byproducts; fermentation; furfural; glucose; hydrolysates; hydrolysis; hydroxymethylfurfural; itaconic acid; lignocellulose;     Show all 13 Subjects
Abstract:
... The ability to produce second-generation itaconic acid by Aspergillus terreus, and the inhibitory effects of hydrolysis by-products on the fermentation were evaluated by cultivation in a synthetic medium containing components usually present in a real hydrolysate broth from lignocellulosic biomasses. The results showed that A. terreus NRRL 1960 can produce itaconic acid and consume xylose complete ...
DOI:
10.1016/j.biortech.2020.123319

9. Selection and application of novel high temperature inducible promoters in the tolerant yeast Candida glycerinogenes

Author:
Wang, Yadi; Lin, Yanping; Lu, Xinyao; Zhuge, Bin; Zong, Hong
Source:
Journal of bioscience and bioengineering 2020 v.130 no.1 pp. 1-5
ISSN:
1389-1723
Subject:
xylose,   etc   ; Candida; Neurospora crassa; Saccharomyces cerevisiae; enzymes; fermentation; fluorescence; genes; promoter regions; temperature; xylitol; yeasts;     Show all 12 Subjects
Abstract:
... High temperature fermentation can substantially reduce the contaminations and condensation costs. Candida glycerinogenes was more resistant to high temperature than Saccharomyces cerevisiae. Quantitative real-time PCR results showed that 7 of 33 candidates served as potential high temperature inducible promoters in C. glycerinogenes. Fluorescence analysis indicated that PCgcwp1 showed the highest ...
DOI:
10.1016/j.jbiosc.2020.02.006

10. Butyric acid as sole product from xylose fermentation by a non-solventogenic Clostridium beijerinckii strain under controlled pH and nutritional conditions

Author:
Fonseca, Bruna Constante; Bortolucci, Jonatã; da Silva, Thalita Marques; dos Passos, Vinícius Fabiano; de Gouvêa, Paula Fagundes; Dinamarco, Taísa Magnani; Reginatto, Valeria
Source:
Bioresource technology reports 2020 v.10 pp. 100426
ISSN:
2589-014X
Subject:
xylose,   etc   ; Clostridium beijerinckii; acetate kinase; acetates; butyric acid; carbon nitrogen ratio; fermentation; genes; lignocellulose; pH;     Show all 10 Subjects
Abstract:
... Fermentative processes allow butyric acid to be produced from renewable substrates, which makes this compound commercially attractive. We have employed the non-solventogenic C. beijerinckii strain Br21 to obtain butyric acid from xylose under different nutritional conditions and controlled pH. Reinforced Clostridium Medium (RCM), which has the lowest C/N ratio, provided the highest butyrate/acetat ...
DOI:
10.1016/j.biteb.2020.100426

11. Application of Maillard reaction product of xylose–pea protein enzymatic hydrolysate in 3D printing

Author:
Zhou, Quancheng; Wang, Meng; Li, Hui; Wang, Sai; Sun, Wanting; Chen, Xuanhong; Dong, Leichao; Ruan, Zheng
Source:
Journal of the science of food and agriculture 2020 v.100 no.7 pp. 2982-2990
ISSN:
0022-5142
Subject:
xylose,   etc   ; Maillard reaction products; food industry; functional foods; hydrolysates; pea protein; physical properties; three-dimensional printing;     Show all 8 Subjects
Abstract:
... BACKGROUND: This paper aims to provide a basis for the application of Maillard reaction products and ideas for 3D printing of functional food. RESULTS: We evaluated the effects of pea protein enzymatic hydrolysate–xylose Maillard reaction products (xMRPs) on the printability of 3D printing materials and the structure using various techniques. It was found that compared with the addition of enzymat ...
DOI:
10.1002/jsfa.10327

12. Effect of furfural addition on validamycin-A production in fermentation of Streptomyces hygroscopicus 5008

Author:
Lu, Zheng-Yan; Zhong, Jian-Jiang
Source:
Process biochemistry 2020 v.92 pp. 43-48
ISSN:
1359-5113
Subject:
xylose,   etc   ; Streptomyces hygroscopicus; antibiotics; enzyme activity; fermentation; furfural; furfuryl alcohol; hemicellulose; hydrogen peroxide; hydrolysates;     Show all 10 Subjects
Abstract:
... Furfural is one of main inhibitors in hemicellulose hydrolysates such as xylose mother liquor, but its positive effect on the production of validamycin-A (VAL-A), a widely used agricultural antibiotic, was interestingly found in fermentation of Streptomyces hygroscopicus 5008. The furfural level in medium up to 1 g/L was effectively converted to furfuryl alcohol and furoic acid by the microorganis ...
DOI:
10.1016/j.procbio.2020.03.002

13. Pretreated Sugarcane Bagasse with Citric Acid Applied in Enzymatic Hydrolysis

Author:
Gomes, Michelle Garcia; dos Santos, Renata Vidal; Barreto, Elisa da Silva; Baffi, Milla Alves; Gurgel, Leandro Vinicius Alves; Baêta, Bruno Eduardo Lobo; Pasquini, Daniel
Source:
Industrial biotechnology 2020 v.16 no.2 pp. 117-124
ISSN:
1931-8421
Subject:
xylose,   etc   ; biotechnology; cellulose; citric acid; enzymatic hydrolysis; enzymes; experimental design; glucose; hemicellulose; lignin; sugarcane bagasse; temperature;     Show all 12 Subjects
Abstract:
... The objective of the study was to evaluate the pretreatment of sugarcane bagasse (SCB) with diluted citric acid solution in a pressurized system and its behavior when submitted to enzymatic hydrolysis using the Cellic® CTec 3 enzyme complex, employing a Doehlert's experimental design (2³), with 5 central points (CP). The operating variables were temperature (T, °C), time (t, min) and percentage by ...
DOI:
10.1089/ind.2019.0039

14. Efficient xylose utilization leads to highest lipid productivity in Candida tropicalis SY005 among six yeast strains grown in mixed sugar medium

Author:
Chattopadhyay, Atrayee; Maiti, Mrinal K.
Source:
Applied microbiology and biotechnology 2020 v.104 no.7 pp. 3133-3144
ISSN:
0175-7598
Subject:
xylose,   etc   ; Candida tropicalis; Trichosporon; enzymes; genes; glucose; hydrolysates; lignocellulose; lipids; metabolism; xylitol; yeasts;     Show all 12 Subjects
Abstract:
... Six local isolates of yeasts were screened for cell mass and lipid production in mixed glucose and xylose medium. Candida tropicalis SY005 and Trichosporon (Apiotrichum) loubieri SY006 showed significant lipid accumulation of 24.6% and 32% (dry cell weight), respectively when grown in medium containing equal mass of both the sugars. SY005 produced relatively higher cell mass of 9.66 gL⁻¹ due to hi ...
DOI:
10.1007/s00253-020-10443-z

15. A stereoselective approach in preparation of γ-lactam precursors for oxazolomycin’s synthesis

Author:
Oleksak, Patrik; Gonda, Jozef; Imrich, Jan; Malinak, David; Lycka, Antonin; Musilek, Kamil
Source:
Tetrahedron 2020 pp. 131111
ISSN:
0040-4020
Subject:
xylose,   etc   ; chelation; chemical bonding; chemical structure; models; organic compounds; stereoselectivity; stereospecificity;     Show all 8 Subjects
Abstract:
... A stereoselective approach to C-3′ methyl attached γ-lactam derivatives as the precursors of pyroglutamate core of oxazolomycins is described. The synthesis started from d-xylose and utilized [3,3]-heterosigmatropic rearrangement. The key lactamization was achieved with intramolecular aldol reaction and ring-closing metathesis. The accomplished stereoselective aldol cyclization was elucidated with ...
DOI:
10.1016/j.tet.2020.131111

16. A study of the residual fermentation sugars influence on an alternative downstream process for first and second-generation lactic acid

Author:
Oliveira, Regiane Alves de; Komesu, Andrea; Vaz Rossell, Carlos Eduardo; Wolf Maciel, Maria Regina; Maciel Filho, Rubens
Source:
Sustainable chemistry and pharmacy 2020 v.15 pp. 100206
ISSN:
2352-5541
Subject:
xylose,   etc   ; distillation; feedstocks; fermentation; glucose; green chemistry; lactic acid; market share; solvents; sucrose;     Show all 10 Subjects
Abstract:
... Lactic acid is an important biomolecule with a substantial market share. Currently, investments in the area are concentrated on new substrates and technologies, in order to boost production and separation performance. Molecular distillation has been recognized as a promising technology in the separation, purification, and concentration of natural products and hence with potential for application i ...
DOI:
10.1016/j.scp.2019.100206

17. Improved production of 3-hydroxypropionic acid in engineered Escherichia coli by rebalancing heterologous and endogenous synthetic pathways

Author:
Lee, Tae-Young; Min, Won-Ki; Kim, Hyo Jin; Seo, Jin-Ho
Source:
Bioresource technology 2020 v.299 pp. 122600
ISSN:
0960-8524
Subject:
xylose,   etc   ; Escherichia coli; aldehyde dehydrogenase; batch fermentation; biochemical pathways; gene overexpression; glucose; glycerol; operon; transcription (genetics);     Show all 10 Subjects
Abstract:
... To improve 3-hydroxypropionic acid (3-HP) production by Escherichia coli, glycerol accumulation needs to be reduced. To accomplish this, we constructed a novel E. coli strain that overexpresses the endogenous aldehyde dehydrogenase gene (puuC) under the control of a strong promoter. The fermentation performance of the engineered strain was significantly improved compared to that of the parental co ...
DOI:
10.1016/j.biortech.2019.122600

18. Optimization of selective acidolysis pretreatment for the valorization of wheat straw by a combined chemical and enzymatic process

Author:
Guo, Jianming; Huang, Kaixuan; Zhang, Shizhong; Xu, Yong
Source:
Journal of chemical technology and biotechnology 2020 v.95 no.3 pp. 694-701
ISSN:
0268-2575
Subject:
xylose,   etc   ; acetic acid; acidolysis; biotechnology; cellulose; crops; enzymatic hydrolysis; glucose; polymers; wheat straw; xylan; xylooligosaccharides;     Show all 12 Subjects
Abstract:
... BACKGROUND: Advances in industrial biotechnology offer potential opportunities for economic utilization of abundantly available agricultural crop waste such as wheat straw, which is of great interest for advancement in xylo‐oligosaccharides (XOS) and fermentable monosaccharides. In the present study, crude XOS and fermentable monosaccharides were sequentially produced by a combined chemical and en ...
DOI:
10.1002/jctb.6251

19. Simultaneous hydrogen and ethanol production in a thermophilic AFBR: a comparative approach between cellulosic hydrolysate single fermentation and the fermentation of glucose and xylose as co-substrates

Author:
Lopes, Helberth Júnnior Santos; Ramos, Lucas Rodrigues; de Menezes, Camila Aparecida; Silva, Edson Luiz
Source:
Cellulose 2020 v.27 no.5 pp. 2599-2612
ISSN:
0969-0239
Subject:
xylose,   etc   ; cellulose; energy; ethanol; ethanol production; fermentation; fluidized beds; glucose; hydrogen; hydrogen production; hydrolysates;     Show all 11 Subjects
Abstract:
... This study evaluated the effect of hydraulic retention time (HRT) on the simultaneous hydrogen and ethanol production in two anaerobic fluidized bed reactors (55 °C) from mono-fermentation of cellulosic hydrolysate (AFBR-C) and the fermentation of glucose and xylose as co-substrates (AFBR-GX). In AFBR-C, the HRT was decreased from 24 to 8 h, while in AFBR-GX, the HRT was decreased from 16 to 0.5 h ...
DOI:
10.1007/s10570-020-03000-4

20. The effect of sodium chloride concentration on the mutarotation and structure of d-xylose in water: Experimental and theoretical investigation

Author:
Li, Zheng; Luo, Yiping; Wang, Xiaoyan; Jiang, Zhicheng; Xu, Shuguang; Hu, Changwei
Source:
Carbohydrate research 2020 v.489 pp. 107941
ISSN:
0008-6215
Subject:
xylose,   etc   ; hemicellulose; moieties; nuclear magnetic resonance spectroscopy; sodium; sodium chloride; stable isotopes;     Show all 7 Subjects
Abstract:
... The effect of NaCl concentration on the structure of d-xylose in H₂O was studied. It was found that NaCl could prolong the equilibrium time between the two main configurations, α-xylopyranose and β-xylopyranose. The proportion of α-xylopyranose was slightly increased in NaCl–H₂O solution than that in H₂O, and the alteration of NaCl on α-xylopyranose and β-xylopyranose was different. Theoretical ca ...
DOI:
10.1016/j.carres.2020.107941