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1. New tools for the new bug Candida auris

Author:
Alexander Lorenz; Nicolas Papon
Source:
Trends in microbiology 2022 v.30 no.3 pp. 203-205
ISSN:
0966-842X
Subject:
yeasts,   etc   ; Candida; pathogens; reverse genetics; virulence;     Show all 5 Subjects
Abstract:
... Candida auris is a recently emerged yeast pathogen of humans causing severe hospital-acquired systemic infections. It is of the utmost importance to understand the genetic and cellular basis of its virulence and pathogenicity. In a recent study, Santana and O’Meara generated forward and reverse genetic tools to manipulate C. auris. ...
DOI:
10.1016/j.tim.2022.01.010

2. Using DMS-MaPseq to uncover the roles of DEAD-box proteins in ribosome assembly

Author:
Xin Liu; Haina Huang; Katrin Karbstein
Source:
Methods 2022 pp. -
ISSN:
1046-2023
Subject:
yeasts,   etc   ; RNA; RNA-binding proteins; ribosomes;     Show all 4 Subjects
Abstract:
... DMS (dimethylsulfate) is a time-tested chemical probe for nucleic acid secondary structure that has recently re-emerged as a powerful tool to study RNA structure and structural changes, by coupling it to high throughput sequencing techniques. This variant, termed DMS-MaPseq, allows for mapping of all RNAs in a cell at the same time. However, if an RNA adopts different structures, for example durin ...
DOI:
10.1016/j.ymeth.2022.05.001

3. A novel yeast‐based screening system for potential compounds that can alleviate human α‐synuclein toxicity

Author:
Anyaporn Sangkaew; Thanaporn Kojornna; Ryoya Tanahashi; Hiroshi Takagi; Chulee Yompakdee
Source:
Journal of applied microbiology 2022 v.132 no.2 pp. 1409-1421
ISSN:
1364-5072
Subject:
yeasts,   etc   ; drugs; dyes; genes; humans; plasmids; toxicity;     Show all 7 Subjects
Abstract:
... AIMS: This study aimed to establish a yeast‐based screening system for potential compounds that can alleviate the toxicity of α‐synuclein (α‐syn), a neuropathological hallmark of Parkinson’s disease, either inhibition of α‐syn aggregation or promotion of ubiquitin‐mediated degradation of α‐syn. METHODS AND RESULTS: A powerful yeast‐based screening assay using the rsp5ᴬ⁴⁰¹ᴱ‐mutant strain, which is ...
DOI:
10.1111/jam.15256

4. Biofilm formation in clinically relevant filamentous fungi: a therapeutic challenge

Author:
Maryam Roudbary; Roya Vahedi-Shahandashti; André Luis Souza dos Santos; Shahla Roudbar Mohammadi; Peyman Aslani; Cornelia Lass-Flörl; Célia F. Rodrigues
Source:
Critical reviews in microbiology 2022 v.48 no.2 pp. 197-221
ISSN:
1549-7828
Subject:
yeasts,   etc   ; biofilm; extracellular matrix; immune system; therapeutics;     Show all 5 Subjects
Abstract:
... Biofilms are highly-organized microbial communities attached to a biotic or an abiotic surface, surrounded by an extracellular matrix secreted by the biofilm-forming cells. The majority of fungal pathogens contribute to biofilm formation within tissues or biomedical devices, leading to serious and persistent infections. The clinical significance of biofilms relies on the increased resistance to co ...
DOI:
10.1080/1040841X.2021.1950121

5. How Cells Deal with the Fluctuating Environment: Autophagy Regulation under Stress in Yeast and Mammalian Systems

Author:
Yuchen Lei; Yuxiang Huang; Xin Wen; Zhangyuan Yin; Zhihai Zhang; Daniel J. Klionsky
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
yeasts,   etc   ; autophagy; energy; homeostasis; mammals; organelles; oxygen;     Show all 7 Subjects
Abstract:
... Eukaryotic cells frequently experience fluctuations of the external and internal environments, such as changes in nutrient, energy and oxygen sources, and protein folding status, which, after reaching a particular threshold, become a type of stress. Cells develop several ways to deal with these various types of stress to maintain homeostasis and survival. Among the cellular survival mechanisms, au ...
DOI:
10.3390/antiox11020304

6. Identification of uncharacterized proteins potentially localized to mitochondria (UPMs) in Saccharomyces cerevisiae using a fluorescent protein unstable in the cytoplasm

Author:
Satoshi Horiuchi; Shotaro Namba; Nozomu Saeki; Ayano Satoh; Hisao Moriya
Source:
Yeast 2022 v.39 no.5 pp. 303-311
ISSN:
0749-503X
Subject:
yeasts,   etc   ; Saccharomyces cerevisiae; fluorescent proteins; mitochondria; prediction;     Show all 5 Subjects
Abstract:
... Eukaryotic cells are composed of organelles, and each organelle contains proteins that play a role in its function. Therefore, the localization of a protein, especially to organelles, is a clue to infer the function of that protein. In this study, we attempted to identify novel mitochondrially localized proteins in the budding yeast Saccharomyces cerevisiae using a fluorescent protein (GFPdeg) tha ...
DOI:
10.1002/yea.3685

7. Linkage reprogramming by tailor-made E3s reveals polyubiquitin chain requirements in DNA-damage bypass

Author:
Sabrina Wegmann; Cindy Meister; Christian Renz; George Yakoub; Hans-Peter Wollscheid; Diane T. Takahashi; Ivan Mikicic; Petra Beli; Helle D. Ulrich
Source:
Molecular cell 2022 v.82 no.8 pp. 1589-1602.e5
ISSN:
1097-2765
Subject:
yeasts,   etc   ; DNA damage; ubiquitin; ubiquitin-protein ligase;     Show all 4 Subjects
Abstract:
... A polyubiquitin chain can adopt a variety of shapes, depending on how the ubiquitin monomers are joined. However, the relevance of linkage for the signaling functions of polyubiquitin chains is often poorly understood because of our inability to control or manipulate this parameter in vivo. Here, we present a strategy for reprogramming polyubiquitin chain linkage by means of tailor-made, linkage- ...
DOI:
10.1016/j.molcel.2022.02.016

8. Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences

Author:
Shiran Barber-Zucker; Vladimir Mindel; Eva Garcia-Ruiz; Jonathan J. Weinstein; Miguel Alcalde; Sarel J. Fleishman
Source:
Journal of the American Chemical Society 2022 v.144 no.8 pp. 3564-3571
ISSN:
1520-5126
Subject:
yeasts,   etc   ; functional diversity; genomics; lignin; peroxidases; prediction;     Show all 6 Subjects
Abstract:
... White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous biocatalysts. VPs are attractive enzymes for research and industrial use but their recombinant production is extremely challenging. To date, only a single VP has been structurally characterized and optimized for recombi ...
DOI:
10.1021/jacs.1c12433

9. The emerging roles of ATG1/ATG13 kinase complex in plants

Author:
Qiuling Wang; Suiwen Hou
Source:
Journal of plant physiology 2022 v.271 pp. 153653
ISSN:
0176-1617
Subject:
yeasts,   etc   ; autophagosomes; autophagy; nutritional status; plant physiology;     Show all 5 Subjects
Abstract:
... Autophagy is a conserved system from yeast to mammals that mediates the degradation and renovation of cellular components. This process is mainly driven by numerous autophagy-related (ATG) proteins. Among these components, the ATG1/ATG13 complex plays an essential role in initiating autophagy, sensing nutritional status signals, recruiting downstream ATG proteins to the autophagosome formation sit ...
DOI:
10.1016/j.jplph.2022.153653

10. A yeast-based biosensor for silver nanoparticle accumulation and cellular dissolution

Author:
Anqi Sun; Wen-Xiong Wang
Source:
Biosensors & bioelectronics 2022 v.205 pp. 114082
ISSN:
0956-5663
Subject:
yeasts,   etc   ; adenine; biological assessment; biomass; biosensors; fluorescence; nanosilver;     Show all 7 Subjects
Abstract:
... Silver nanoparticles (AgNPs) are now being increasingly applied in many fields, thus it is critical to quantify these nanoparticles as well as their cellular dissolution for biological assessment. In this study, we developed a novel technique to quantify the concentration of ultra-small AgNPs based principally on the finding that dissolved Ag⁺ decreased the Zn²⁺ directed fluorescence increase of a ...
DOI:
10.1016/j.bios.2022.114082

11. In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States

Author:
Oran Melanker; Pierre Goloubinoff; Gideon Schreiber
Source:
Journal of molecular biology 2022 pp. 167627
ISSN:
0022-2836
Subject:
yeasts,   etc   ; Escherichia coli; molecular biology; mutants; proteinases; uracil;     Show all 6 Subjects
Abstract:
... Natural evolution is driven by random mutations that improve fitness. In vitro evolution mimics this process, however, on a short time-scale and is driven by the given bait. Here, we used directed in vitro evolution of a random mutant library of Uracil glycosylase (eUNG) displayed on yeast surface to select for binding to chaperones GroEL, DnaK + DnaJ + ATP (DnaKJ) or E. coli cell extract (CE), us ...
DOI:
10.1016/j.jmb.2022.167627

12. Probiotic properties of yeasts in traditional fermented foods and beverages

Author:
Jyoti Prakash Tamang; Sonam Lama
Source:
Journal of applied microbiology 2022 v.132 no.5 pp. 3533-3542
ISSN:
1364-5072
Subject:
yeasts,   etc   ; Saccharomyces cerevisiae; commercialization; fermentation; lactic acid; probiotics;     Show all 6 Subjects
Abstract:
... The interest in potentiality and functionality of probiotic yeasts from fermented foods has increased drastically over the years. In many fermented foods and beverages, lactic acid bacteria and yeasts exist synergistically by stimulating their growth and survival. Probiotic strains of lactic acid bacteria are more widely studied than potential probiotic yeasts. Saccharomyces cerevisiae variety bou ...
DOI:
10.1111/jam.15467

13. Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae

Author:
Xin-Yi Tao; Yang-Chen Lin; Feng-Qing Wang; Qing-Hai Liu; Yu-Shu Ma; Min Liu; Dong-Zhi Wei
Source:
Biotechnology letters 2022 v.44 no.4 pp. 571-580
ISSN:
0141-5492
Subject:
yeasts,   etc   ; Saccharomyces cerevisiae; biosynthesis; biotechnology; cosmetics; mitochondria; sesquiterpenoids;     Show all 7 Subjects
Abstract:
... Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain (0.66 mg/L) using genome-integrated patchoulol synthase, indicating that mitochondria compartmentati ...
DOI:
10.1007/s10529-022-03240-3

14. Amyloid protein‐induced sequestration of the eukaryotic ribosome: effect of stoichiometry and polyphenolic inhibitors

Author:
Sehnaz Ferdosh; Senjuti Banerjee; Jayshree Singh; Chandana Barat
Source:
FEBS letters 2022 v.596 no.9 pp. 1190-1202
ISSN:
0014-5793
Subject:
yeasts,   etc   ; Alzheimer disease; amyloid; myricetin; ribosomes; rosmarinic acid; stoichiometry;     Show all 7 Subjects
Abstract:
... Alzheimer's disease (AD) is characterized by the appearance of neurofibrillary tangles comprising of the Tau protein and aggregation of amyloid‐β peptides (Aβ 1–40 and Aβ 1–42). A concomitant loss of the ribosomal population is also observed in AD‐affected neurons. Our studies demonstrate that, similarly to Tau protein aggregation, in vitro aggregation of Aβ peptides in the vicinity of the yeast 8 ...
DOI:
10.1002/1873-3468.14308

15. Chaperones directly and efficiently disperse stress-triggered biomolecular condensates

Author:
Haneul Yoo; Jared A.M. Bard; Evgeny V. Pilipenko; D. Allan Drummond
Source:
Molecular cell 2022 v.82 no.4 pp. 741-755.e11
ISSN:
1097-2765
Subject:
yeasts,   etc   ; condensates; heat shock response; heat stress; luciferase; toxicity;     Show all 6 Subjects
Abstract:
... Stresses such as heat shock trigger the formation of protein aggregates and the induction of a disaggregation system composed of molecular chaperones. Recent work reveals that several cases of apparent heat-induced aggregation, long thought to be the result of toxic misfolding, instead reflect evolved, adaptive biomolecular condensation, with chaperone activity contributing to condensate regulatio ...
DOI:
10.1016/j.molcel.2022.01.005

16. Dynamical modeling for non-Gaussian data with high-dimensional sparse ordinary differential equations

Author:
Muye Nanshan; Nan Zhang; Xiaolei Xun; Jiguo Cao
Source:
Computational statistics & data analysis 2022 v.173 pp. 107483
ISSN:
0167-9473
Subject:
yeasts,   etc   ; cell cycle; data analysis; data collection; stock exchange;     Show all 5 Subjects
Abstract:
... Ordinary differential equations (ODE) have been widely used for modeling dynamical complex systems. For high-dimensional ODE models where the number of differential equations is large, it remains challenging to estimate the ODE parameters and to identify the sparse structure of the ODE models. Most existing methods exploit the least-square based approach and are only applicable to Gaussian observa ...
DOI:
10.1016/j.csda.2022.107483

17. Efficiency of pre‐process anoxia treatment on quality of fresh‐cut pineapples cv. ‘Phulae’ during storage

Author:
Suriyan Supapvanich; Chairat Techavuthiporn
Source:
International journal of food science & technology 2022 v.57 no.3 pp. 1679-1687
ISSN:
0950-5423
Subject:
yeasts,   etc   ; ascorbic acid; food science; hypoxia; pineapples; weight loss;     Show all 6 Subjects
Abstract:
... The aim of this study was to investigate the competency of pre‐process anoxia treatment on physicochemical quality of fresh‐cut pineapples cv. ‘Phulae’ during storage. The fruits were kept in anoxia atmosphere (N₂) for 16 h before processing. The fruits were processed immediately after treatment (G1) and after storage at 13 °C for 6 days (G2). Anoxia treatment delayed the weight loss and had no ef ...
DOI:
10.1111/ijfs.15533

18. Nucleosome Positioning on Large Tandem DNA Repeats of the ‘601’ Sequence Engineered in Saccharomyces cerevisiae

Author:
Astrid Lancrey; Alexandra Joubert; Evelyne Duvernois-Berthet; Etienne Routhier; Saurabh Raj; Agnès Thierry; Marta Sigarteu; Loic Ponger; Vincent Croquette; Julien Mozziconacci; Jean-Baptiste Boulé
Source:
Journal of molecular biology 2022 v.434 no.7 pp. 167497
ISSN:
0022-2836
Subject:
yeasts,   etc   ; DNA; Saccharomyces cerevisiae; molecular biology; nucleosomes; nucleotide sequences;     Show all 6 Subjects
Abstract:
... The artificial 601 DNA sequence is often used to constrain the position of nucleosomes on a DNA molecule in vitro. Although the ability of the 147 base pair sequence to precisely position a nucleosome in vitro is well documented, application of this property in vivo has been explored only in a few studies and yielded contradictory conclusions. Our goal in the present study was to test the ability ...
DOI:
10.1016/j.jmb.2022.167497

19. The diverse effects of yeast on the aroma of non-sulfite added white wines throughout aging

Author:
M. Denat; I. Ontañón; A. Querol; V. Ferreira
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.158 pp. 113111
ISSN:
0023-6438
Subject:
yeasts,   etc   ; Lewis acids; aldehydes; fermentation; grapes; odors; secondary metabolites;     Show all 7 Subjects
Abstract:
... Semi-synthetic must containing standard nutrients, a phenolic and aromatic fraction extracted from Albariño grapes and synthetic precursors of 3-mercaptohexanol (MOH) and 4-mercapto-4-pentan-2-one (MMP) were fermented with three different selected commercial S. cerevisiae yeasts. Wines were subjected to anoxic aging at 50 °C for 1, 2, 5 and 8 weeks, and their volatile composition was comprehensive ...
DOI:
10.1016/j.lwt.2022.113111

20. The influence of two yeast strains on fermentation and flavour composition of cider

Author:
Hasan Tangüler; Hüseyin Erten
Source:
Flavour and fragrance journal 2022 v.37 no.3 pp. 144-153
ISSN:
0882-5734
Subject:
yeasts,   etc   ; Saccharomyces cerevisiae; ciders; ethanol; fermentation; flavor; inoculum; odors;     Show all 8 Subjects
Abstract:
... Two commercial dry strains of Saccharomyces cerevisiae and their different inoculum levels were used to carry out cider fermentation to assess their impact on the quality and volatile flavour composition of cider. Analyses indicated that the composition and production of flavour compounds of ciders are generally significantly affected by the strains and inoculation levels. Additionally, the Uvafer ...
DOI:
10.1002/ffj.3693