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yeasts, etc ; Candida; pathogens; reverse genetics; virulence; Show all 5 Subjects
Abstract:
... Candida auris is a recently emerged yeast pathogen of humans causing severe hospital-acquired systemic infections. It is of the utmost importance to understand the genetic and cellular basis of its virulence and pathogenicity. In a recent study, Santana and O’Meara generated forward and reverse genetic tools to manipulate C. auris. ...
... DMS (dimethylsulfate) is a time-tested chemical probe for nucleic acid secondary structure that has recently re-emerged as a powerful tool to study RNA structure and structural changes, by coupling it to high throughput sequencing techniques. This variant, termed DMS-MaPseq, allows for mapping of all RNAs in a cell at the same time. However, if an RNA adopts different structures, for example durin ...
yeasts, etc ; drugs; dyes; genes; humans; plasmids; toxicity; Show all 7 Subjects
Abstract:
... AIMS: This study aimed to establish a yeast‐based screening system for potential compounds that can alleviate the toxicity of α‐synuclein (α‐syn), a neuropathological hallmark of Parkinson’s disease, either inhibition of α‐syn aggregation or promotion of ubiquitin‐mediated degradation of α‐syn. METHODS AND RESULTS: A powerful yeast‐based screening assay using the rsp5ᴬ⁴⁰¹ᴱ‐mutant strain, which is ...
Maryam Roudbary; Roya Vahedi-Shahandashti; André Luis Souza dos Santos; Shahla Roudbar Mohammadi; Peyman Aslani; Cornelia Lass-Flörl; Célia F. Rodrigues
yeasts, etc ; biofilm; extracellular matrix; immune system; therapeutics; Show all 5 Subjects
Abstract:
... Biofilms are highly-organized microbial communities attached to a biotic or an abiotic surface, surrounded by an extracellular matrix secreted by the biofilm-forming cells. The majority of fungal pathogens contribute to biofilm formation within tissues or biomedical devices, leading to serious and persistent infections. The clinical significance of biofilms relies on the increased resistance to co ...
yeasts, etc ; autophagy; energy; homeostasis; mammals; organelles; oxygen; Show all 7 Subjects
Abstract:
... Eukaryotic cells frequently experience fluctuations of the external and internal environments, such as changes in nutrient, energy and oxygen sources, and protein folding status, which, after reaching a particular threshold, become a type of stress. Cells develop several ways to deal with these various types of stress to maintain homeostasis and survival. Among the cellular survival mechanisms, au ...
yeasts, etc ; Saccharomyces cerevisiae; fluorescent proteins; mitochondria; prediction; Show all 5 Subjects
Abstract:
... Eukaryotic cells are composed of organelles, and each organelle contains proteins that play a role in its function. Therefore, the localization of a protein, especially to organelles, is a clue to infer the function of that protein. In this study, we attempted to identify novel mitochondrially localized proteins in the budding yeast Saccharomyces cerevisiae using a fluorescent protein (GFPdeg) tha ...
yeasts, etc ; DNA damage; ubiquitin; ubiquitin-protein ligase; Show all 4 Subjects
Abstract:
... A polyubiquitin chain can adopt a variety of shapes, depending on how the ubiquitin monomers are joined. However, the relevance of linkage for the signaling functions of polyubiquitin chains is often poorly understood because of our inability to control or manipulate this parameter in vivo. Here, we present a strategy for reprogramming polyubiquitin chain linkage by means of tailor-made, linkage- ...
yeasts, etc ; functional diversity; genomics; lignin; peroxidases; prediction; Show all 6 Subjects
Abstract:
... White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous biocatalysts. VPs are attractive enzymes for research and industrial use but their recombinant production is extremely challenging. To date, only a single VP has been structurally characterized and optimized for recombi ...
yeasts, etc ; autophagosomes; autophagy; nutritional status; plant physiology; Show all 5 Subjects
Abstract:
... Autophagy is a conserved system from yeast to mammals that mediates the degradation and renovation of cellular components. This process is mainly driven by numerous autophagy-related (ATG) proteins. Among these components, the ATG1/ATG13 complex plays an essential role in initiating autophagy, sensing nutritional status signals, recruiting downstream ATG proteins to the autophagosome formation sit ...
yeasts, etc ; adenine; biological assessment; biomass; biosensors; fluorescence; nanosilver; Show all 7 Subjects
Abstract:
... Silver nanoparticles (AgNPs) are now being increasingly applied in many fields, thus it is critical to quantify these nanoparticles as well as their cellular dissolution for biological assessment. In this study, we developed a novel technique to quantify the concentration of ultra-small AgNPs based principally on the finding that dissolved Ag⁺ decreased the Zn²⁺ directed fluorescence increase of a ...
yeasts, etc ; Escherichia coli; molecular biology; mutants; proteinases; uracil; Show all 6 Subjects
Abstract:
... Natural evolution is driven by random mutations that improve fitness. In vitro evolution mimics this process, however, on a short time-scale and is driven by the given bait. Here, we used directed in vitro evolution of a random mutant library of Uracil glycosylase (eUNG) displayed on yeast surface to select for binding to chaperones GroEL, DnaK + DnaJ + ATP (DnaKJ) or E. coli cell extract (CE), us ...
yeasts, etc ; Saccharomyces cerevisiae; commercialization; fermentation; lactic acid; probiotics; Show all 6 Subjects
Abstract:
... The interest in potentiality and functionality of probiotic yeasts from fermented foods has increased drastically over the years. In many fermented foods and beverages, lactic acid bacteria and yeasts exist synergistically by stimulating their growth and survival. Probiotic strains of lactic acid bacteria are more widely studied than potential probiotic yeasts. Saccharomyces cerevisiae variety bou ...
yeasts, etc ; Saccharomyces cerevisiae; biosynthesis; biotechnology; cosmetics; mitochondria; sesquiterpenoids; Show all 7 Subjects
Abstract:
... Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain (0.66 mg/L) using genome-integrated patchoulol synthase, indicating that mitochondria compartmentati ...
yeasts, etc ; Alzheimer disease; amyloid; myricetin; ribosomes; rosmarinic acid; stoichiometry; Show all 7 Subjects
Abstract:
... Alzheimer's disease (AD) is characterized by the appearance of neurofibrillary tangles comprising of the Tau protein and aggregation of amyloid‐β peptides (Aβ 1–40 and Aβ 1–42). A concomitant loss of the ribosomal population is also observed in AD‐affected neurons. Our studies demonstrate that, similarly to Tau protein aggregation, in vitro aggregation of Aβ peptides in the vicinity of the yeast 8 ...
yeasts, etc ; condensates; heat shock response; heat stress; luciferase; toxicity; Show all 6 Subjects
Abstract:
... Stresses such as heat shock trigger the formation of protein aggregates and the induction of a disaggregation system composed of molecular chaperones. Recent work reveals that several cases of apparent heat-induced aggregation, long thought to be the result of toxic misfolding, instead reflect evolved, adaptive biomolecular condensation, with chaperone activity contributing to condensate regulatio ...
yeasts, etc ; cell cycle; data analysis; data collection; stock exchange; Show all 5 Subjects
Abstract:
... Ordinary differential equations (ODE) have been widely used for modeling dynamical complex systems. For high-dimensional ODE models where the number of differential equations is large, it remains challenging to estimate the ODE parameters and to identify the sparse structure of the ODE models. Most existing methods exploit the least-square based approach and are only applicable to Gaussian observa ...
yeasts, etc ; ascorbic acid; food science; hypoxia; pineapples; weight loss; Show all 6 Subjects
Abstract:
... The aim of this study was to investigate the competency of pre‐process anoxia treatment on physicochemical quality of fresh‐cut pineapples cv. ‘Phulae’ during storage. The fruits were kept in anoxia atmosphere (N₂) for 16 h before processing. The fruits were processed immediately after treatment (G1) and after storage at 13 °C for 6 days (G2). Anoxia treatment delayed the weight loss and had no ef ...
yeasts, etc ; DNA; Saccharomyces cerevisiae; molecular biology; nucleosomes; nucleotide sequences; Show all 6 Subjects
Abstract:
... The artificial 601 DNA sequence is often used to constrain the position of nucleosomes on a DNA molecule in vitro. Although the ability of the 147 base pair sequence to precisely position a nucleosome in vitro is well documented, application of this property in vivo has been explored only in a few studies and yielded contradictory conclusions. Our goal in the present study was to test the ability ...
yeasts, etc ; Lewis acids; aldehydes; fermentation; grapes; odors; secondary metabolites; Show all 7 Subjects
Abstract:
... Semi-synthetic must containing standard nutrients, a phenolic and aromatic fraction extracted from Albariño grapes and synthetic precursors of 3-mercaptohexanol (MOH) and 4-mercapto-4-pentan-2-one (MMP) were fermented with three different selected commercial S. cerevisiae yeasts. Wines were subjected to anoxic aging at 50 °C for 1, 2, 5 and 8 weeks, and their volatile composition was comprehensive ...
yeasts, etc ; Saccharomyces cerevisiae; ciders; ethanol; fermentation; flavor; inoculum; odors; Show all 8 Subjects
Abstract:
... Two commercial dry strains of Saccharomyces cerevisiae and their different inoculum levels were used to carry out cider fermentation to assess their impact on the quality and volatile flavour composition of cider. Analyses indicated that the composition and production of flavour compounds of ciders are generally significantly affected by the strains and inoculation levels. Additionally, the Uvafer ...