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Comparison of three different C₁₈ HPLC columns with different particle sizes for the optimization of aflatoxins analysis

Medina, A., Magan, N.
Journal of chromatography 2012 v.889-890 pp. 138-143
aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, agar, corn meal, cost analysis, culture media, derivatization, detection limit, hazelnuts, high performance liquid chromatography, particle size, solvents, sucrose, yeasts
In this work we compared the performance of chromatography columns with particles of 5 and 3μm with the new 2.7μm solid core particles for the analysis of aflatoxins B1, G1, B2, and G2 using trifluoroacetic acid pre-column derivatization. Three different columns have been used and chromatographic parameters as retention time, resolution, limit of detection (LOD), limit of quantification (LOQ) were obtained from all of them and compared. The results show that comparing with the traditional columns, shorter columns (100mm×4.6mm) with the new solid core particles are suitable for the analysis of these mycotoxins and allowed the reduction of the analysis time by 45.5% and 33.3% with respect to columns with particle size 5μm (150mm×4.6mm) and 3μm (150mm×4.6mm) respectively, without any detrimental effect on performance. This leads to the reduction of the analysis costs by saving on organic solvents and increasing the total number of analyses per day. The capability of these columns for analyzing samples, in different culture media, was assessed by analyzing different samples from: yeasts extract sucrose medium, corn meal agar medium and fresh hazelnut media.