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Determination of 17 macrolide antibiotics and avermectins residues in meat with accelerated solvent extraction by liquid chromatography–tandem mass spectrometry

Tao, Yanfei, Yu, Gang, Chen, Dongmei, Pan, Yuanhu, Liu, Zhenli, Wei, Huimin, Peng, Dapeng, Huang, Lingli, Wang, Yulian, Yuan, Zonghui
Journal of chromatography 2012 v.897 pp. 64-71
animal-based foods, antibiotic residues, automation, avermectins, liquid chromatography, meat, roxithromycin, solvents, tandem mass spectrometry, temperature
A method has been developed for simultaneous determination of 17 kinds of macrolide antibiotics and avermectins residues in animal origin foods. Samples were extracted with acetonitrile-methanol using accelerated solvent extraction (ASE) instrument. Parameters such as extraction temperature and pressure were investigated by a fractional factorial design (FFD) and the selected extraction (60°C, 1500psi for 10min in two cycles) was most effective. High correlation coefficients (r>0.999) of 17 macrolide antibiotics and avermectins were obtained within their respective linear ranges (2–400μg/kg) using roxithromycin as internal standard. The recoveries of them were above 75% at different spiked levels in various samples. Using ASE the method was featured as short extraction times, reduction use of extraction solvent, high extraction yields, with high level of automation.