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Determination of 17 macrolide antibiotics and avermectins residues in meat with accelerated solvent extraction by liquid chromatography–tandem mass spectrometry

Author:
Tao, Yanfei, Yu, Gang, Chen, Dongmei, Pan, Yuanhu, Liu, Zhenli, Wei, Huimin, Peng, Dapeng, Huang, Lingli, Wang, Yulian, Yuan, Zonghui
Source:
Journal of chromatography 2012 v.897 pp. 64-71
ISSN:
1570-0232
Subject:
animal-based foods, antibiotic residues, automation, avermectins, liquid chromatography, meat, roxithromycin, solvents, tandem mass spectrometry, temperature
Abstract:
A method has been developed for simultaneous determination of 17 kinds of macrolide antibiotics and avermectins residues in animal origin foods. Samples were extracted with acetonitrile-methanol using accelerated solvent extraction (ASE) instrument. Parameters such as extraction temperature and pressure were investigated by a fractional factorial design (FFD) and the selected extraction (60°C, 1500psi for 10min in two cycles) was most effective. High correlation coefficients (r>0.999) of 17 macrolide antibiotics and avermectins were obtained within their respective linear ranges (2–400μg/kg) using roxithromycin as internal standard. The recoveries of them were above 75% at different spiked levels in various samples. Using ASE the method was featured as short extraction times, reduction use of extraction solvent, high extraction yields, with high level of automation.
Agid:
1004093