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The aggregation of Tyr-Phe dipeptide and Val-Tyr-Val tripeptide in aqueous solution and in the presence of SDS and PEO–PPO–PEO triblock copolymer: Fluorescence spectroscopic studies

Author:
James, Jilly, Mandal, Asit Baran
Source:
Journal of colloid and interface science 2011 v.360 no.2 pp. 600-605
ISSN:
0021-9797
Subject:
aqueous solutions, composite polymers, fluorescence, micelles, peptides, spectral analysis, spectroscopy, tyrosine
Abstract:
The micelle formation of Tyr-Phe dipeptide and Val-Tyr-Val tripeptide has been studied for the first time. The aggregation numbers were determined for both the peptides in aqueous solution and in the presence of SDS and PEO–PPO–PEO triblock copolymer additive environments. The results obtained by steady state and time-resolved fluorescence spectroscopic techniques are in good agreement with each other. The higher value of aggregation number confirms the formation of mixed micelles. The fluorescence lifetime of tyrosine in various micellar and mixed micellar systems were also determined. The decreased lifetime values with the quencher suggested the dynamic nature of the quenching process. However, the possibility of static quenching cannot be ruled out. The accessibility of DPC quencher was found to be more in dipeptide than tripeptide.
Agid:
1007076