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Affinity electromembrane with covalently coupled heparin for thrombin adsorption

Author:
Wang, Jun, Wu, Guisen, Shi, Wei, Liu, Xin, Ruan, Changqing, Xue, Maoqiang, Ge, Dongtao
Source:
Journal of membrane science 2013 v.428 pp. 70-77
ISSN:
0376-7388
Subject:
adsorption, artificial membranes, desorption, electrical conductivity, electrical treatment, heparin, nylon, thrombin
Abstract:
A new composite membrane with electrical conductivity and reactive groups (–COOH) was synthesized by copolymerizing pyrrole-3-carboxylic acid and pyrrole onto nylon membrane via the template method. The ligand, heparin, was covalently coupled onto the composite membrane by activating with N-hydroxysuccinimide and 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and modified with ethylenediamine. The as-synthesized affinity electromembrane exhibited a uniform conductivity value of 3.7×10⁻⁷S/cm and the content of heparin was approximately 1.897mg/g membrane. By applying an electrical stimulation (−0.5V) to the affinity electromembrane, the adsorption capacity and rate were greatly enhanced. Elution of protein showed desorption ratio was up to 92.7% using 2.0M NaSCN solution as the desorption agent. To investigate the performance of dynamic adsorption of heparin-coupled affinity electromembrane, a membrane cartridge to which electrical stimulation can be applied was designed. The result of dynamic separation showed that the time of breakthrough was delayed from 22min in the absence of electrical stimulation to 31min in the presence of electrical stimulation.
Agid:
1019689