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Aequorin-expressing yeast emits light under electric control
- Vilanova, Cristina, Hueso, Ãngeles, Palanca, Carles, Marco, Guillem, Pitarch, Miguel, Otero, Eduardo, Crespo, Juny, Szablowski, Jerzy, Rivera, Sara, DomÃnguez-EscribÃ , Laura, Navarro, Emilio, Montagud, Arnau, de CÃ³rdoba, Pedro FernÃ¡ndez, GonzÃ¡lez, Asier, AriÃ±o, JoaquÃn, Moya, AndrÃ©s, UrchueguÃa, Javier, Porcar, Manuel
- Journal of biotechnology 2011 v.152 no.3 pp. 93-95
- Scyphozoa, aequorin, biotechnology, electrical treatment, electrodes, generators (equipment), genetically modified organisms, interphase, luminescence, synthetic biology, yeasts
- In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.