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Aequorin-expressing yeast emits light under electric control

Author:
Vilanova, Cristina, Hueso, Ángeles, Palanca, Carles, Marco, Guillem, Pitarch, Miguel, Otero, Eduardo, Crespo, Juny, Szablowski, Jerzy, Rivera, Sara, Domínguez-Escribà, Laura, Navarro, Emilio, Montagud, Arnau, de Córdoba, Pedro Fernández, González, Asier, Ariño, Joaquín, Moya, Andrés, Urchueguía, Javier, Porcar, Manuel
Source:
Journal of biotechnology 2011 v.152 no.3 pp. 93-95
ISSN:
0168-1656
Subject:
Scyphozoa, aequorin, biotechnology, electrical treatment, electrodes, generators (equipment), genetically modified organisms, interphase, luminescence, synthetic biology, yeasts
Abstract:
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
Agid:
102489