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Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants

Author:
Deng, M., Schneider, W.L., Allison, R.F.
Source:
Virus research 2006 v.122 no.1-2 pp. 171
Subject:
Nicotiana benthamiana, transgenic plants, gene expression, transgenes, Cowpea chlorotic mottle virus, coat proteins, Brome mosaic virus, virus replication, genetic recombination, RNA
Abstract:
Viral transgenes designed to provide resistance to specific plant viruses frequently consist of the coat protein gene and a contiguous 3' untranslated region (3'UTR) of viral origin. In many RNA viruses the viral 3'UTR establishes a recognition and initiation site for viral RNA replication. Thus the transgenic transcript may contain a functional virus replication site. Experiments were designed to determine if a challenging virus would recognize this replication site on a nuclear derived transcript and synthesize the complementary RNA. These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced. In these experiments the replication complex of Brome Mosaic bromovirus recognized the transgenic transcript derived from a Cowpea Chlorotic Mottle bromovirus transgene. The resulting RNA may contribute to RNA recombination events.
Agid:
1039
Handle:
10113/1039