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Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants

Deng, M., Schneider, W.L., Allison, R.F.
Virus research 2006 v.122 no.1-2 pp. 171
Nicotiana benthamiana, transgenic plants, gene expression, transgenes, Cowpea chlorotic mottle virus, coat proteins, Brome mosaic virus, virus replication, genetic recombination, RNA
Viral transgenes designed to provide resistance to specific plant viruses frequently consist of the coat protein gene and a contiguous 3' untranslated region (3'UTR) of viral origin. In many RNA viruses the viral 3'UTR establishes a recognition and initiation site for viral RNA replication. Thus the transgenic transcript may contain a functional virus replication site. Experiments were designed to determine if a challenging virus would recognize this replication site on a nuclear derived transcript and synthesize the complementary RNA. These data demonstrate that upon infection by a virus that recognizes the viral replication site, a full-length complement of the transgenic transcript is produced. In these experiments the replication complex of Brome Mosaic bromovirus recognized the transgenic transcript derived from a Cowpea Chlorotic Mottle bromovirus transgene. The resulting RNA may contribute to RNA recombination events.