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A highly sensitive and selective turn-on fluorogenic and colorimetric sensor based on pyrene-functionalized magnetic nanoparticles for Hg²⁺ detection and cell imaging

Chen, Ling, Zheng, Baozhan, Guo, Yong, Du, Juan, Xiao, Dan, Bo, Lin
Talanta 2013 v.117 pp. 338-344
Fourier transform infrared spectroscopy, X-radiation, X-ray diffraction, blood serum, colorimetry, confocal laser scanning microscopy, fluorescence, hydrolysis, image analysis, iron oxides, magnetic separation, mercury, monitoring, nanoparticles, scanning electron microscopy, transmission electron microscopy
In this paper, a colorimetric and “turn-on” fluorescent sensor (Py–Si–Fe₃O₄@SiO₂ NPs) for Hg²⁺ detection was designed with pyrene derivative covalently grafted onto the surface of magnetic core/shell Fe₃O₄@SiO₂ nanoparticles using the silanol hydrolysis approach. The Py–Si–Fe₃O₄@SiO₂ inorganic–organic hybrid material was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray power diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and fluorescence emission. The results of fluorescence spectra showed that the resultant multifunctional nanoparticles exhibited selective turn-on type fluorescence enhancement with Hg²⁺. In addition, the presence of magnetic Fe₃O₄ nanoparticles in the sensor Py–Si–Fe₃O₄@SiO₂ NPs would also facilitate the magnetic separation of Hg²⁺–Py–Si–Fe₃O₄@SiO₂ from the solution. The as-prepared chemosensor was also successfully applied to detect Hg²⁺ in environmental water samples and serum sample. Results from confocal laser scanning microscopy experiments demonstrated that this chemosensor was cell permeable and can be used as a fluorescent probe for monitoring Hg²⁺ in living cells.