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A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly

Author:
Sciaranghella, Gaia, Lakhashe, Samir K., Ayash-Rashkovsky, Mila, Mirshahidi, Saied, Siddappa, Nagadenahalli B., Novembre, Francis J., Velu, Vijayakumar, Amara, Rama Rao, Zhou, Chenghui, Li, Sufen, Li, Zhongxia, Frankel, Fred R., Ruprecht, Ruth M.
Source:
Vaccine 2011 v.29 no.3 pp. 476-486
ISSN:
0264-410X
Subject:
Listeria monocytogenes, Macaca mulatta, bacteria, cell-mediated immunity, humoral immunity, vaccines
Abstract:
Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag, which encodes SIVmac239 gag. Intragastric (i.g.) administration of 3×10¹² bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.
Agid:
1109872