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A novel chitosan based antimalarial drug delivery against Plasmodium berghei infection

Tripathy, Satyajit, Mahapatra, Santanu Kar, Chattopadhyay, Sourav, Das, Sabyasachi, Dash, Sandeep Kumar, Majumder, Subrata, Pramanik, Panchanan, Roy, Somenath
Acta tropica 2013 v.128 no.3 pp. 494-503
DNA, DNA damage, Plasmodium berghei, antioxidants, chitosan, chloroquine, enzymes, flow cytometry, gelation, inflammation, lymphocytes, mice, nanoparticles, oxidative stress, parasitemia, parasites, polymers, sodium tripolyphosphate, therapeutics
Chitosan is a natural polysaccharide that has attracted significant scientific interest during the last two decades and chitosan based nanodrug delivery systems seem to be a hopeful and viable strategy for improving disease treatment. This study aims to evaluate the potency of the polymer based nanochloroquine in application for attenuation of Plasmodium berghei infection in Swiss mice and effectiveness against the parasite induced oxidative stress and deoxyribo nucleic acid (DNA) damage in lymphocytes. Nanoparticle was prepared by ionotropic gelation between chitosan and sodium tripolyphosphate. The chloroquine was treated by the actual drug content of effective nanochloroquine and the nanodrug was charged with its effective dose for fifteen days, after successive infection development in Swiss mice. Gimsa staining of thin smear and flow cytometry analysis was pursued to reveal the parasitemia. Different oxidative markers, inflammatory markers, antioxidant enzymes level and also lymphocytic deoxyribo nucleic acid damage study were performed. The present study reveals the potency of the nanodrug which has been found as more prospective than only chloroquine treatment to combat the parasite infection, oxidative stress as well as inflammation and DNA damage. From the study, we conclude this nanodrug may be applicable as potent therapeutic agent than only chloroquine.