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Fibrillation of transferrin

Author:
Booyjz̈sen, Claire, Scarff, Charlotte A., Moreton, Ben, Portman, Ian, Scrivens, James H., Costantini, Giovanni, Sadler, Peter J.
Source:
Biochimica et biophysica acta 2012 v.1820 no.3 pp. 427-436
ISSN:
0304-4165
Subject:
Alzheimer disease, amyloid, aqueous solutions, atomic force microscopy, blood serum, deformation, dye binding, electron microscopy, gases, gel chromatography, humans, hydrophilicity, light scattering, mass spectrometry, mica, transferrin
Abstract:
BACKGROUND: The nature of fibrillar deposits from aqueous solutions of human serum and recombinant human transferrin on mica and carbon-coated formvar surfaces has been investigated. METHODS AND RESULTS: Atomic force microscopy showed that the deposition of recombinant transferrin onto the hydrophilic surface of mica resulted in the formation of a monolayer-thick film composed of conformationally-strained flattened protein molecules. Elongated fibres developed on top of this layer and appeared to be composed of single proteins or small clusters thereof. Monomeric and dimeric transferrins were separated by gel permeation chromatography and their states of aggregation confirmed by mass spectrometry and dynamic light scattering. Transmission electron-microscopy showed that dimeric transferrin, but not monomeric transferrin, deposited on carbon-coated formvar grids forms rounded (circular) structures ca. 250nm in diameter. Small transferrin fibrils ca. 250nm long appeared to be composed of smaller rounded sub-units. Synchrotron radiation-circular dichroism and, Congo red and thioflavin-T dye-binding experiments suggested that transferrin aggregation in solution does not involve major structural changes to the protein or formation of classical β-sheet amyloid structures. Collisional cross sections determined via ion mobility–mass spectrometry showed little difference between the overall protein shapes of apo- and holo-transferrin in the gas phase. GENERAL SIGNIFICANCE: The possibility that transferrin deformation and aggregation are involved in neurological disorders such as Parkinson's and Alzheimer's disease is discussed. This article is part of a Special Issue entitled Transferrins: Molecular mechanisms of iron transport and disorders.
Agid:
1119607