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Facile detection of photogenerated reactive oxygen species in TiO2 nanoparticles suspension using colorimetric probe-assisted spectrometric method
- Kim, Choonsoo, Park, Hee-jin, Cha, Seoeun, Yoon, Jeyong
- Chemosphere 2013 v.93 pp. 2011-2015
- centrifugation, colorimetry, ionic strength, nanoparticles, oxygen, reactive oxygen species, spectroscopy, tetrazolium, titanium dioxide
- This present study reports that reactive oxygen species (ROS), which mainly consist of hydroxyl (OH) and superoxide (O2-) radicals generated by the photocatalysis of TiO2 nanoparticles (TiO2 NPs) in suspension, can be quantitatively measured by the colorimetric probe-assisted spectrometric method without any interference by the TiO2 NPs. The interference effect of the TiO2 NPs with UV–vis spectroscopy was excluded by the selective separation of p-nitrosodimethylaniline (RNO) and tetrazolium salt (XTT) (probe compounds for OH and O2-, respectively) from the TiO2 NPs in the suspension by centrifugation at a specified ionic strength adjusted with phosphate buffer (KH2PO4/NaOH, PB) solution. This colorimetric probe-assisted spectrometric method was also successfully applied to measure the steady-state production of photogenerated ROS in a TiO2 NPs suspension.