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Identification of immunological expressed sequence tags in the mealworm beetle Tenebrio molitor
- Dobson, Adam J., Johnston, Paul R., Vilcinskas, Andreas, Rolff, Jens
- Journal of insect physiology 2012 v.58 no.12 pp. 1556-1561
- Staphylococcus aureus, Tenebrio molitor, antibiotics, antimicrobial peptides, antimicrobial properties, ecology, enzymes, expressed sequence tags, immune response, immune system, invertebrates, larvae, messenger RNA, models
- Understanding the evolutionary ecology of immune responses to persistent infection could provide fundamental insight into temporal dynamics or interactive mechanisms that could be co-opted for antibiotic treatment regimes. Additionally, identification of novel molecules involved in these processes could provide novel compounds for biotechnological development. The beetle Tenebrio molitor displays a high level of induced antimicrobial activity coincident with persistent immuno-resistant Staphylococcus aureus, and is the first invertebrate model for persistent infection. Here we present expressed sequence tags (ESTs) detected by suppression-subtraction hybridization of Tenebrio larvae after infection with S. aureus. Amongst others, we identified mRNAs coding for various oxidative enzymes and two antimicrobial peptides. These ESTs provide a foundation for mechanistic study of Tenebrio’s immune system.