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Magnetic solid-phase extraction based on diphenyl functionalization of Fe₃O₄ magnetic nanoparticles for the determination of polycyclic aromatic hydrocarbons in urine samples

Bianchi, F., Chiesi, V., Casoli, F., Luches, P., Nasi, L., Careri, M., Mangia, A.
Journal of chromatography 2012 v.1231 pp. 8-15
X-ray diffraction, X-ray photoelectron spectroscopy, biphenyl, chromatography, infrared spectroscopy, iron oxides, magnetic properties, nanoparticles, polycyclic aromatic hydrocarbons, scanning electron microscopy, solid phase extraction, surface area, transmission electron microscopy, urine
Superparamagnetic Fe₃O₄ diphenyl nanoparticles were prepared according to a solvothernal procedure and characterized by X-ray diffraction, infrared spectroscopy, surface area measurements, scanning electron microscopy, X-ray photoelectron spectroscopy and transmission electron microscopy. The magnetic phases present in the nanoparticle samples were analyzed by thermomagnetic analysis and the samples’ magnetic properties were studied by vibrating sample magnetometry. The resulting nanoparticles having an average diameter of 200nm were then used as solid-phase extraction sorbent for the determination of polycyclic aromatic hydrocarbons in urine samples. Method validation proved the feasibility of the developed beads for the quantitation of the investigated analytes at trace levels obtaining lower limit of quantitation values in the ng/l range. A good precision with coefficients of variations always lower than 15% was obtained. Finally, the superior extraction performance of the synthesized nanoparticles with respect to commercially available beads was proved.