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A double-modification strategy for enhancing charge density of mono-sized beads for facilitated refolding of like-charged protein

Author:
Yang, Chun-Yan, Li, Ming, Dong, Xiao-Yan, Sun, Yan
Source:
Journal of chromatography 2013 v.1299 pp. 85-93
ISSN:
0021-9673
Subject:
adsorption, bovine serum albumin, chromatography, ionic strength, lysozyme, molecular weight, salt tolerance
Abstract:
We have previously found that addition of like-charge media in a refolding system can greatly enhance protein refolding yield, and the media of higher charge density (q) are favorable for facilitating protein refolding. Herein, a double-modification strategy was developed to increase the charge density of mono-sized poly(glycidyl methacrylate) (PGMA) microspheres (0.8μm). The PGMA beads were firstly modified with poly(ethylenimine) of molecular weight 60,000 (PEIL) or 1200 (PEIS) and then further modified with 2-diethylaminoethylchloride (DEAE) or PEIS. The charge density and bovine serum albumin (BSA) adsorption density (QBSA) of the beads were significantly increased by the double modification. The QBSA of the double-modified beads with the highest q (824μmol/g) reached 36.3mg/g, over 35% higher than that of the single-modified beads (26.7mg/g) with the highest q (300μmol/g) that a single modification could achieve. The double-modified beads with different ligand structures and charge densities were used for facilitating the refolding of like-charged lysozyme. Lysozyme refolding yield with the single-modified beads of the highest q (300μmol/g) was 60% at a critical bead concentration (cc) of 100mg/mL. By contrast, the refolding yields with the double-modified beads of q>500μmol/g were 70%, and the refolding yields with the double-modified beads of q≥650μmol/g could even reach 70% at a cc of 40mg/mL. This indicates that the double-modified beads of high q values could enhance like-charged protein refolding more significantly than the single-modified beads at a low bead utilization. The facilitating effect of the like-charged beads was independent of the ligand structure. The beads of higher q values showed lower salt sensitivity in protein refolding, and were expected beneficial for use in refolding buffers of higher ionic strengths. The sequential modification strategy for enhancing charge density would help develop more efficient media for protein adsorption and protein refolding applications.
Agid:
1130917