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Cytotoxic cardenolide glycosides from the root of Reevesia formosana

Author:
Chang, Hsun-Shuo, Chiang, Michael Y., Hsu, Hsing-Yu, Yang, Cheng-Wei, Lin, Chu-Hung, Lee, Shiow-Ju, Chen, Ih-Sheng
Source:
Phytochemistry 2013 v.87 pp. 86-95
ISSN:
0031-9422
Subject:
Malvaceae, X-ray diffraction, cardiac glycosides, cytotoxicity, fractionation, inhibitory concentration 50, spectroscopy, sugars
Abstract:
Bioassay-guided fractionation of the root tissue of Reevesia formosana led to isolation of 13 cardenolide glycosides, reevesiosides A–I and epi-reevesiosides F–I. Their structures were determined by means of spectroscopic analysis and single-crystal X-ray diffraction was performed using reevesioside A. Reevesioside A, reevesioside F, and epi-reevesioside F displayed especially potent cytotoxicity against the MCF-7 and NCI-H460 cancer cell lines, with IC50 values of 63±2 and 19±1, 72±8 and 20±0, and 34±6 and 10±1nM, respectively. Identification of the sugar constituents and unusual 18,20-epoxide cardenolide glycosides are described herein. Cardiac glycosides were previously unknown in the Sterculiaceae family.
Agid:
1135568