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Characterization of the porcine p65 subunit of NF-κB and its association with virus antibody levels
- Li, Hegang, Ma, Guojian, Gui, Duan, Zhao, Shuanping, Wang, Pan, He, Kongwang, Wang, Xuemin, Ruan, Jinxue, Cao, Jiyue, Yang, Shulin, Li, Kui
- Molecular immunology 2011 v.48 no.6-7 pp. 914-923
- Classical swine fever virus, Suid herpesvirus 1, adaptive immunity, antibodies, endothelial cells, fibroblasts, gene expression, liver, macrophages, messenger RNA, reproduction, single nucleotide polymorphism, small intestine, spleen, swine, transcription factor NF-kappa B, viruses
- NF-κB p65 subunit plays important roles in controlling both innate and adaptive immunity. Here we report the characterization of porcine NF-κB p65 subunit (pp65). pp65 shows high similarity to other mammalian counterparts. pp65 mRNA expression was mainly observed in lung, spleen, liver and small intestine. Furthermore, overexpression of pp65 activates NF-κB in porcine endothelial cell line PIEC, porcine alveolar macrophages cell line 3D4/21 and porcine primary fetal fibroblasts. A COOH-terminal truncation derivative of pp65 (pp65RHD) has been identified as a specific transdominant inhibitor of NFκB. Association study was performed on the selected SNP and indel. The results revealed that the SNP BglI was significantly associated (P<0.05) with pig reproduction and respiratory syndrome virus antibody level (PRRSV-AB) (0 day and 17 days), the classical swine fever virus (CSFV) antibody blocking rates (CSFV-AB) (0 day and 17 days) and pseudorabies virus antibody level (PRV-AB) (0 day and 32 days).