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Characterization of the porcine p65 subunit of NF-κB and its association with virus antibody levels

Li, Hegang, Ma, Guojian, Gui, Duan, Zhao, Shuanping, Wang, Pan, He, Kongwang, Wang, Xuemin, Ruan, Jinxue, Cao, Jiyue, Yang, Shulin, Li, Kui
Molecular immunology 2011 v.48 no.6-7 pp. 914-923
Classical swine fever virus, Suid herpesvirus 1, adaptive immunity, antibodies, endothelial cells, fibroblasts, gene expression, liver, macrophages, messenger RNA, reproduction, single nucleotide polymorphism, small intestine, spleen, swine, transcription factor NF-kappa B, viruses
NF-κB p65 subunit plays important roles in controlling both innate and adaptive immunity. Here we report the characterization of porcine NF-κB p65 subunit (pp65). pp65 shows high similarity to other mammalian counterparts. pp65 mRNA expression was mainly observed in lung, spleen, liver and small intestine. Furthermore, overexpression of pp65 activates NF-κB in porcine endothelial cell line PIEC, porcine alveolar macrophages cell line 3D4/21 and porcine primary fetal fibroblasts. A COOH-terminal truncation derivative of pp65 (pp65RHD) has been identified as a specific transdominant inhibitor of NFκB. Association study was performed on the selected SNP and indel. The results revealed that the SNP BglI was significantly associated (P<0.05) with pig reproduction and respiratory syndrome virus antibody level (PRRSV-AB) (0 day and 17 days), the classical swine fever virus (CSFV) antibody blocking rates (CSFV-AB) (0 day and 17 days) and pseudorabies virus antibody level (PRV-AB) (0 day and 32 days).