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HSP90 is crucial for regulation of LAT expression in activated T cells

Author:
Hayashi, Keitaro, Kamikawa, Yuichiro
Source:
Molecular immunology 2011 v.48 no.6-7 pp. 941-946
ISSN:
0161-5890
Subject:
T-lymphocytes, gene expression, heat shock proteins, humans, messenger RNA, protein kinases, regulator genes, signal transduction
Abstract:
T cell response initiated by engagement of T cell receptor (TCR) is dependent on signal transduction events composed of protein kinases and adaptor proteins. However, the molecular mechanism for gene expression of these proteins is not entirely understood. Here we identified Heat Shock Protein 90 (HSP90) as an essential regulator for gene expression of Linker for activation of T cells (LAT) in primarily activated human T cells. Primarily activated T cells continuously synthesized LAT protein and treatment of cells with 17-AAG, a pharmacological inhibitor of HSP90, decreased LAT protein level following reduction of LAT mRNA. Furthermore, promoter activity of LAT gene was dramatically inhibited by 17-AAG. These results reveal a novel role of HSP90 as a positive regulator for expression of LAT gene in activated T cells.
Agid:
1138101