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Characterization of potential MnP producing bacteria and its metabolic products during decolourisation of synthetic melanoidins due to biostimulatory effect of d-xylose at stationary phase

Yadav, Sangeeta, Chandra, Ram, Rai, Vibhuti
Process biochemistry 2011 v.46 no.9 pp. 1774-1784
Cronobacter sakazakii, Raoultella planticola, bacteria, biochemical oxygen demand, chemical oxygen demand, decolorization, electrospray ionization mass spectrometry, manganese peroxidase, melanoidins, microbial growth, new products, pH, pollutants, temperature, wastewater
Sucrose–aspartic acid Maillard product (SAA-MP) is the major colourant of distillery effluent as environmental pollutant due to its recalcitrant nature. Three potential manganese peroxidase (MnP) producing bacteria were screened for higher SAA-MP tolerance (3200mg/l) and characterized as Bacillus species (IITRM7, FJ581030), Raoultella planticola (IITRM15, GU329705) and Enterobacter sakazakii (IITRM16, FJ581031). The consortium of these bacteria showed maximum decolourisation (60%) of SAA-MP (2400mg/l) in modified GPYM medium at optimized nutrient, pH (7.0±0.2), shaking speed (180rpm) and temperature (35±2°C) after 144h incubation. The addition of d-xylose enhanced the decolourisation of SAA-MP from 60 to 75% along with reduction of BOD and COD. The electrospray ionization-mass spectrum (ESI-MS) analysis showed removal of various compounds after bacterial growth and decolourisation of SAA-MP. Formation of new products showed metabolization of SAA-MP. Thus this consortium might be useful for decolourisation of industrial wastewater containing high concentration of melanoidins. d-xylose could be used as biostimulator for this consortium during decolourisation of SAA-MP.