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Ameliorative effect of 20-OH ecdysone on streptozotocin induced oxidative stress and β-cell damage in experimental hyperglycemic rats

Sundaram, Ramalingam, Naresh, Rajendran, Shanthi, Palanivelu, Sachdanandam, Panchanatham
Process biochemistry 2012 v.47 no.12 pp. 2072-2080
alanine transaminase, animal disease models, antioxidant activity, antioxidants, ascorbic acid, aspartate transaminase, blood glucose, catalase, citrates, creatinine, diabetes mellitus, ecdysone, glibenclamide, glutathione, glutathione peroxidase, glutathione transferase, hyperglycemia, hypoglycemic agents, insulin, intraperitoneal injection, islets of Langerhans, kidneys, liver, oxidative stress, pH, rats, streptozotocin, superoxide dismutase, tissues, urea, vitamin E
The present study was to evaluate the effects of 20-OH ecdysone on hyperglycemia mediated oxidative stress in streptozotocin induced diabetic rats. Diabetes was induced in experimental rats by single intraperitoneal injection of STZ (45mg/kg b.w.) dissolved in 0.1mol/L citrate buffer (pH 4.5). Diabetic rats exhibited increased blood glucose with significant decrease in plasma insulin levels. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and the levels of non-enzymic antioxidants vitamin C, vitamin E and reduced glutathione (GSH) were decreased while increases in the levels of LPO markers were observed in liver and kidney tissues of diabetic rats. Moreover, hepatic markers (aspartate aminotransferase and alanine aminotransferase) and renal markers (urea, creatinine) were significantly increased in diabetic rats as compared to control rats. Upon treatment with 20-OH ecdysone to diabetic rats showed significant ameliorative effects on all the biochemical parameters studied. Biochemical findings were supported by histological studies. These results indicated that 20-OH ecdysone exerts a protective action on pancreatic beta cell function and overcomes oxidative stress through its hypoglycemic potential. The effect produced by the 20-OH ecdysone on various parameters was comparable to that of glibenclamide – an antidiabetic drug.