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Sphingobacterium nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco

Liu, Jie, Yang, Ling-Ling, Xu, Chuan-Kun, Xi, Jia-Qin, Yang, Fa-Xiang, Zhou, Feng, Zhou, Yu, Mo, Ming-He, Li, Wen-Jun
International journal of systematic and evolutionary microbiology 2012 v.62 no.Pt 8 pp. 1809-1813
DNA, Nicotiana tabacum, Sphingobacterium, bacteria, endophytes, fatty acids, genes, leaves, new species, nucleotide sequences, pH, phylogeny, ribosomal RNA, sequence homology, sodium chloride, tobacco, China
A novel Gram-negative strain, designated M-SX103T, was isolated from a fresh leaf of Nicotiana tabacum Linn. collected at Yuxi City, Yunnan province, China, and subjected to a polyphasic taxonomic study. Growth occurred at 10–40 °C, at pH 6.0–9.0 and with 0–3 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids of strain M-SX103T were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The G+C content of the genomic DNA was 40.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Sphingobacterium, sharing 95.4–89.7 % sequence similarity with type strains of members of the genus Sphingobacterium; it was most closely related to Sphingobacterium composti T5-12T. On the basis of the taxonomic evidence, a novel species, Sphingobacterium nematocida sp. nov., is proposed. The type strain is M-SX103T ( = JCM 17339T = CCTCC AB 2010390T).