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Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011

Author:
Hijazin, Muaz, Prenger-Berninghoff, Ellen, Sammra, Osama, Alber, Jörg, Lämmler, Christoph, Kämpfer, Peter, Glaeser, Stefanie P., Busse, Hans-Jürgen, Hassan, Abdulwahed Ahmed, Abdulmawjood, Amir, Zschöck, Michael
Source:
International journal of systematic and evolutionary microbiology 2012 v.62 no.Pt 9 pp. 2201-2205
ISSN:
1466-5034
Subject:
Arcanobacterium haemolyticum, Arcanobacterium phocae, Gram-positive bacteria, dogs, fatty acids, genes, menaquinones, otitis externa, phosphatidylcholines, ribosomal RNA
Abstract:
A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from otitis externa of a dog. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium haemolyticum (97.2 %), Arcanobacterium hippocoleae (96.5 %) and Arcanobacterium phocae (96.4 %). The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile contained the major lipids phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unidentified phospholipid (PL2). Major fatty acids were C14 : 0, C16 : 0, C18 : 0, C18 : 1ω9c and C18 : 2ω6,9c/anteiso-C18 : 0 (detected as a summed feature). C10 : 0 and C12 : 0 were present in minor amounts. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified in the novel species Arcanobacterium canis sp. nov. The type strain of Arcanobacterium canis is P6775T ( = CCM 7958T = CCUG 61573T = CIP 110339T). An emended description of the genus Arcanobacterium is also provided.
Agid:
1181116