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Molecular and phenotypic analyses reveal the non-identity of the Phaeobacter gallaeciensis type strain deposits CIP 105210T and DSM 17395
- Buddruhs, Nora, Pradella, Silke, Göker, Markus, Päuker, Orsola, Pukall, Rüdiger, Spröer, Cathrin, Schumann, Peter, Petersen, Jörn, Brinkhoff, Thorsten
- International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 11 pp. 4340-4349
- cell culture, desorption, genes, internal transcribed spacers, ionization, matrix-assisted laser desorption-ionization mass spectrometry, microorganisms, nucleic acid hybridization, plasmids, ribosomal RNA, secondary metabolites, sequence analysis, France, Germany
- The marine genus Phaeobacter currently comprises six species, some of which were intensively studied mainly due to their ability to produce secondary metabolites. The type strain of the type species, Phaeobacter gallaeciensis BS107T, has been deposited at several public culture collections worldwide. Based on differences in plasmid profiles, we detected that the alleged P. gallaeciensis type strains deposited at the Collection Institute Pasteur (CIP; Paris, France) as CIP 105210 and at the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany) as DSM 17395 are not identical. To determine the identity of these strains, we conducted DNA–DNA hybridization, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), 16S rRNA gene and internal transcribed spacer (ITS) sequence analyses, as well as physiological experiments. Based on the detailed 16S rRNA gene reanalysis we showed that strain CIP 105210 most likely corresponds to the original P. gallaeciensis type strain BS107T. In contrast, the Phaeobacter strain DSM 17395 exhibits a much closer affiliation to Phaeobacter inhibens DSM 16374T ( = T5T) and should thus be allocated to this species. The detection of the dissimilarity of strains CIP 105210T and DSM 17395 will influence future comparative studies within the genus Phaeobacter.