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Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae

Author:
Lee, Learn-Han, Cheah, Yoke-Kqueen, Sidik, Shiran Mohd, Xie, Qing-Yi, Tang, Yi-Li, Lin, Hai-Peng, Mutalib, Nurul-Syakima Ab, Hong, Kui
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 1 pp. 241-248
ISSN:
1466-5034
Subject:
Calidifontibacter, DNA, Demetria, Dermacoccus, Kytococcus, fatty acids, galactose, glucose, glycolipids, menaquinones, microbiology, nucleotide sequences, peptidoglycans, phospholipids, phylogeny, ribosomal RNA, sequence homology, soil, Antarctic region
Abstract:
Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9 % 16S rRNA gene sequence similarity), Branchiibius (95.7 %), Dermacoccus (94.4–95.3 %), Calidifontibacter (94.6 %), Luteipulveratus (94.3 %), Yimella (94.2 %) and Kytococcus (93.1 %). Cells were irregular cocci and short rods. The peptidoglycan type was A4α with an l-Lys–l-Ser–d-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17 : 0 (41.97 %), anteiso-C17 : 1ω9c (32.16 %) and iso-C16 : 0 (7.68 %). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T ( = CGMCC 4.6864T = DSM 24617T).
Agid:
1181288