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Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae

Lee, Learn-Han, Cheah, Yoke-Kqueen, Sidik, Shiran Mohd, Xie, Qing-Yi, Tang, Yi-Li, Lin, Hai-Peng, Mutalib, Nurul-Syakima Ab, Hong, Kui
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 1 pp. 241-248
Calidifontibacter, DNA, Demetria, Dermacoccus, Kytococcus, fatty acids, galactose, glucose, glycolipids, menaquinones, microbiology, nucleotide sequences, peptidoglycans, phospholipids, phylogeny, ribosomal RNA, sequence homology, soil, Antarctic region
Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9 % 16S rRNA gene sequence similarity), Branchiibius (95.7 %), Dermacoccus (94.4–95.3 %), Calidifontibacter (94.6 %), Luteipulveratus (94.3 %), Yimella (94.2 %) and Kytococcus (93.1 %). Cells were irregular cocci and short rods. The peptidoglycan type was A4α with an l-Lys–l-Ser–d-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17 : 0 (41.97 %), anteiso-C17 : 1ω9c (32.16 %) and iso-C16 : 0 (7.68 %). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T ( = CGMCC 4.6864T = DSM 24617T).