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Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana

Author:
Jeong, Sang Hyeon, Park, Moon Su, Jin, Hyun Mi, Lee, Kangseok, Park, Woojun, Jeon, Che Ok
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 1 pp. 332-338
ISSN:
1466-5034
Subject:
DNA, Flavobacteriaceae, bacteria, fatty acids, lipid content, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, salt tolerance, sediments, sequence homology, sodium chloride, South Korea
Abstract:
A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterium, designated strain SC17T, was isolated from sediment of a tidal flat of Suncheon bay in South Korea. Cells were halotolerant, catalase- and oxidase-positive and non-motile rods. Growth of strain SC17T was observed at 5–40 °C (optimum, 25–30 °C), at pH 6.0–8.5 (optimum, pH 7.0) and in the presence of 1–8 % (w/v) NaCl (optimum, 1–2 %). The major cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH), iso-C17 : 0 3-OH, iso-C15 : 1 G and anteiso-C15 : 0. The polar lipid content consisted of phosphatidylethanolamine and unidentified amino lipids and lipids. The G+C content of the genomic DNA was 46.4 mol% and the only respiratory quinone detected was menaquinone-6 (MK-6). Phylogenetic inference based on 16S rRNA gene sequences showed that strain SC17T formed a distinct phyletic lineage within the family Flavobacteriaceae and was most closely related to members of the genera Gaetbulibacter and Tamlana with 95.0–95.8 % sequence similarity. On the basis of phenotypic and molecular features, strain SC17T represents a novel genus of the family Flavobacteriaceae, for which the name Aestuariibaculum suncheonense gen. nov., sp. nov. is proposed. The type strain is SC17T ( = KACC 16186T = JCM 17789T). Emended descriptions of the genera Gaetbulibacter and Tamlana are also proposed.
Agid:
1181304