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Cellulomonas soli sp. nov. and Cellulomonas oligotrophica sp. nov., isolated from soil

Hatayama, Kouta, Esaki, Kouji, Ide, Teruhiko
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 1 pp. 60-65
Cellulomonas, DNA, chemotaxonomy, galactose, genes, glucose, glutamic acid, lipids, mannose, menaquinones, microbiology, nucleic acid hybridization, nucleotide sequences, ornithine, peptidoglycans, phylogeny, rhamnose, ribosomal RNA, soil, Japan
Two novel bacterial strains, designated Kc1T and Kc5T, were isolated from soil in Japan. Cells of the novel strains were Gram-reaction-positive, aerobic or facultatively anaerobic, motile rods. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belonged to the genus Cellulomonas. The 16S rRNA gene sequences of strains Kc1T and Kc5T showed closest similarity to that of Cellulomonas terrae DB5T (98.1 % and 98.4 % similarity, respectively), and the 16S rRNA gene similarity between the two novel strains was 97.8 %. In both strains, the major menaquinone was MK-9(H4), the predominant polar lipids were diphosphatidylglycerol and phosphatidylinositol mannosides, and the peptidoglycan contained ornithine and glutamic acid. Cell-wall sugars were identified as rhamnose, galactose and mannose in strain Kc1T and rhamnose and glucose in strain Kc5T. The DNA G+C contents of strains Kc1T and Kc5T were 73.6 mol% and 75.8 mol%, respectively. Based on the chemotaxonomic and physiological data and the results of DNA–DNA hybridizations, the two strains represent two novel species within the genus Cellulomonas, for which the names Cellulomonas soli sp. nov. (type strain Kc1T = DSM 24484T = JCM 17535T) and Cellulomonas oligotrophica sp. nov. (type strain Kc5T = DSM 24482T = JCM 17534T) are proposed.