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Asanoa siamensis sp. nov., isolated from soil from a temperate peat swamp forest

Author:
Niemhom, Nantawan, Suriyachadkun, Chanwit, Tamura, Tomohiko, Thawai, Chitti
Source:
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 1 pp. 66-71
ISSN:
1466-5034
Subject:
DNA, alanine, fatty acids, forests, glucose, glutamic acid, mannose, menaquinones, microbiology, national parks, nucleic acid hybridization, nucleotide sequences, peat, peptidoglycans, phosphatidylethanolamines, phylogeny, rhamnose, ribosomal RNA, sequence homology, soil, spores, swamps, xylose, Thailand
Abstract:
A novel actinomycete strain, PS7-2T, which produced spore chains borne on the tips of short sporophores, was isolated from soil collected from a temperate peat swamp forest in Phu-Sang National Park, Phayao province, Thailand. The isolate contained glutamic acid, glycine, alanine, 3-hydroxy-diaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars were glucose, mannose, rhamnose and xylose, and the major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The predominant menaquinones were MK-10(H8) and MK-9(H8) and the predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.3 mol%. On the basis of 16S rRNA gene sequence analysis, strain PS7-2T clustered with members of the genus Asanoa and appeared most closely related to the type strains of Asanoa hainanensis (99.5 % sequence similarity), Asanoa iriomotensis (99.0 %), Asanoa ishikariensis (98.9 %) and Asanoa ferruginea (98.5 %). DNA–DNA hybridizations and some physiological and biochemical properties indicated that strain PS7-2T could be readily distinguished from its closest phylogenetic relatives. Based on the phenotypic and genotypic evidence and DNA–DNA relatedness values, strain PS7-2T represents a novel species in the genus Asanoa, for which the name Asanoa siamensis sp. nov. is proposed; the type strain is PS7-2T ( = BCC 41921T = NBRC 107932T).
Agid:
1181318