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Chryseobacterium rigui sp. nov., isolated from an estuarine wetland

Park, Sung-Joon, Choi, Jung-Hye, Cha, Chang-Jun
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 3 pp. 1062-1067
Chryseobacterium, DNA, bacteria, chemotaxonomy, fatty acids, nucleic acid hybridization, nucleotide sequences, pH, phylogeny, polyamines, ribosomal RNA, rivers, sequence homology, sodium chloride, wetlands
A bacterium, designated strain CJ16T, was isolated from the estuarine wetland of the Han River. Cells of the isolate were yellow-pigmented, Gram-stain-negative, non-motile and rod-shaped. Growth of strain CJ16T was observed in TSB at 5–37 °C (optimum 30 °C), at pH 5.0–9.0 (optimum pH 6.0) and with 0–3 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CJ16T was most closely related to Chryseobacterium hagamense KCTC 22545T (97.2 % 16S rRNA gene sequence similarity). Genomic relatedness based on DNA–DNA hybridization between strain CJ16T and C. hagamense KCTC 22545T was 23 % (strain CJ16T as probe) and 19 % (strain KCTC 22545T as probe). Chemotaxonomic analysis revealed that strain CJ16T possessed MK-6 as the major isoprenoid quinone and sym-homospermidine as the predominant polyamine. The predominant fatty acids were iso-C15 : 0 (26.9 %), iso-C17 : 0 3-OH (16.8 %) and summed feature 9 (comprising C16 : 0 10-methyl and/or iso-C17 : 1ω9c; 10.5 %). The DNA G+C content of strain CJ16T was 37.9 mol%. Based on phenotypic, genotypic and phylogenetic studies, strain CJ16T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium rigui sp. nov. is proposed. The type strain is CJ16T ( = KACC 16560T = JCM 18078T).