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Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella

Tsubouchi, Taishi, Shimane, Yasuhiro, Mori, Kozue, Miyazaki, Masayuki, Tame, Akihiro, Uematsu, Katsuyuki, Maruyama, Tadashi, Hatada, Yuji
International journal of systematic and evolutionary microbiology 2013 v.63 no.Pt 4 pp. 1390-1395
DNA, Rhodobacteraceae, bacteria, nucleotide sequences, pH, phylogeny, ribosomal RNA, sediments, sequence homology, sodium chloride
A Gram-stain-negative, aerobic, heterotrophic and salt-tolerant bacterium, designated strain LL-001T, was isolated from a deep subseafloor sediment in Japanese waters. Cells were non-motile rods and colonies were smooth, convex, circular and vermilion. The conditions for growth were 15–35 °C, pH 5.5–7.5 and 1–8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain LL-001T belonged to the genus Loktanella within the family Rhodobacteraceae of the class Alphaproteobacteria. 16S rRNA gene sequence similarity between strain LL-001T and members of the genus Loktanella was 94.5–98.5 %; the highest sequence similarity was with Loktanella hongkongensis UST950701-009PT. DNA–DNA relatedness between strain LL-001T and L. hongkongensis UST950701-009PT was 41.5–43.6 %. The DNA G+C content of strain LL-001T was 69.3 mol%. On the basis of biochemical features and 16S rRNA gene sequence comparison, strain LL-001T is considered to represent a novel species of the genus Loktanella, for which the name Loktanella cinnabarina sp. nov. is proposed. The type strain is LL-001T ( = JCM 18161T = CECT 8072T). The description of the genus Loktanella is also emended.