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Sphingobacterium paludis sp. nov., isolated from wetland soil

Feng, Hao, Zeng, Yanhua, Huang, Yili
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 10 pp. 3453-3458
DNA, Gram-negative bacteria, Sphingobacterium, chemotaxonomy, fatty acids, nucleotide sequences, pH, phylogeny, ribosomal RNA, sequence analysis, sodium chloride, wetland soils, wetlands, China
A novel Gram-stain-negative bacteria, designated S37T, was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37T were aerobic, non-motile rods. Growth occurred at 10–37 °C (optimum, 25 °C), pH 5.0–9.7 (optimum, pH 7.5) and with 0–6 % (w/v) NaCl (optimum, 0.5 %). Based on 16S rRNA gene sequence analysis, strain S37T was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24T (95.78 %). The major fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium paludis sp. nov. (type strain S37T = CGMCC 1.12801T = NBRC 110386T) is proposed.