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Thermomonas carbonis sp. nov., isolated from the soil of a coal mine

Wang, Liang, Zheng, Shixue, Wang, Dan, Wang, Lu, Wang, Gejiao
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 11 pp. 3631-3635
DNA, Thermomonas, coal, fatty acids, genes, microbiology, phosphatidylethanolamines, phylogeny, ribosomal RNA, soil
Strain GZ436T was Gram-stain-negative, aerobic, non-motile, rod-shaped and isolated from the soil of a coal mine. 16S rRNA gene phylogenetic analysis showed that this strain clustered with Thermomonas brevis LMG 21746T (97.5 %), Thermomonas haemolytica A50-7-3T (96.3 %), Thermomonas koreensis KCTC 12540T (96.4 %), Thermomonas hydrothermalis SGM-6T (95.5 %) and Thermomonas fusca LMG 21737T (95.1 %). The major isoprenoid quinone was Q-8. The DNA G+C content was 67 mol%. Strain GZ436T contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unknown aminophospholipid, an unknown phospholipid and an unknown lipid as the major polar lipids. The predominant cellular fatty acids (>5 %) were iso-C15 : 0, iso-C11 : 0, iso-C11 : 0 3-OH, iso-C17 : 1ω9c, C16 : 0 and summed feature 3. The DNA–DNA relatedness value between strain GZ436T and T. brevis LMG 21746T was 54±0.4 %. According to phenotypic and phylogenetic characteristics, strain GZ436T represents a novel species of the genus Thermomonas, for which the name Thermomonas carbonis sp. nov. is proposed. The type strain is GZ436T ( = CCTCC AB 2013364T = KCTC 42013T).