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Streptomyces panaciradicis sp. nov., a β-glucosidase-producing bacterium isolated from ginseng rhizoplane

Lee, Hyo-Jin, Cho, Geon-Yeong, Chung, Sang-Ho, Whang, Kyung-Sook
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 11 pp. 3816-3820
Panax, Streptomyces capoamus, Streptomyces coeruleorubidus, Streptomyces curacoi, bacteria, fatty acids, genes, glucose, mannose, pH, peptidoglycans, phylogeny, quinones, rhizoplane, ribose, ribosomal RNA, sodium chloride, spores
A Gram-staining-positive actinobacterium, designated strain 1MR-8T, was isolated from the rhizoplane of ginseng and its taxonomic status was determined using a polyphasic approach. The isolate formed long chains of spores that were straight, cylindrical and smooth-surfaced. Strain 1MR-8T grew at 10–37 °C (optimum 28 °C), whilst no growth was observed at 45 °C. The pH range for growth was 4.0–11.0 (optimum pH 6.0–8.0) and the NaCl range for growth was 0–7 % (w/v) with optimum growth at 1 % (w/v). Strain 1MR-8T had cell-wall peptidoglycans based on ll-diaminopimelic acid. Glucose, mannose and ribose were the whole-cell sugars. The predominant isoprenoid quinones were MK-9 (H4), MK-9 (H6) and MK-9 (H8) and the major fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequencing studies showed that the novel strain was closely related to the type strains of Streptomyces caeruleatus GIMN4T, Streptomyces curacoi NRRL B-2901T, Streptomyces capoamus JCM 4734T and Streptomyces coeruleorubidus NBRC 12761T with similarities of 98.8 %. However, DNA–DNA relatedness, as well as physiological and biochemical analyses, showed that strain 1MR-8T could be differentiated from its closest phylogenetic relatives. It is proposed that this strain should be classified as a representative of a novel species of the genus Streptomyces, with the suggested name Streptomyces panaciradicis sp. nov. The type strain is 1MR-8T ( = KACC 17632T = NBRC 109811T).