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Chitinophaga jiangningensis sp. nov., a mineral-weathering bacterium

Wang, Qi, Cheng, Cheng, He, Lin-Yan, Huang, Zhi, Sheng, Xia-Fang
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 1 pp. 260-265
Chitinophaga, DNA, bacteria, fatty acids, menaquinones, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, polyamines, ribosomal RNA, sequence homology, China
A Gram-stain-negative, rod-shaped bacterial strain, JN53T, was isolated from the surfaces of weathered rock (potassic trachyte) from Nanjing, Jiangsu Province, PR China. Strain JN53T grew optimally at 30 °C, pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JN53T belonged to the genus Chitinophaga in the family Chitinophagaceae. It was most closely related to Chitinophaga terrae KP01T (97.3 % 16S rRNA gene sequence similarity), Chitinophaga eiseniae YC6729T (96.3 %). Strain JN53T contained MK-7 as the major menaquinone and homospermidine as the major polyamine. The main fatty acids of strain JN53T were iso-C15 : 0, C16 : 1ω5c, C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3), iso-C17 : 0 3-OH, C16 : 0, iso-C15 : 0 3-OH and C16 : 0 3-OH. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids and unknown lipids. The total DNA G+C content of strain JN53T was 49.7 mol%. The low level of DNA–DNA relatedness to other species of the genus Chitinophaga and the many phenotypic properties that distinguished strain JN53T from recognized species of this genus demonstrated that isolate JN53T should be classified as representing a novel species of the genus Chitinophaga, for which the name Chitinophaga jiangningensis sp. nov. is proposed. The type strain is JN53T ( = CCTCC AB 2013166T = JCM 19354T).