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Sphingomonas yantingensis sp. nov., a mineral-weathering bacterium isolated from purplish paddy soil

Huang, Jing, Huang, Zhi, Zhang, Zhen-Dong, He, Lin-Yan, Sheng, Xia-Fang
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 3 pp. 1030-1034
DNA, Sphingomonas mali, Sphingomonas pruni, bacteria, fatty acids, nucleotide sequences, pH, paddy soils, phylogeny, polyamines, ribosomal RNA, ubiquinones, China
A novel type of mineral-weathering bacterium was isolated from purplish soils collected from Yanting (Sichuan, south-western China). Cells of strain 1007T were Gram-stain-negative and rod-shaped, motile and yellow-pigmented. The isolate was strictly aerobic, catalase- and oxidase-positive, and grew optimally at 28-30 °C and pH 6.0-7.0. The genomic DNA G+C content of strain 1007T was 67±0.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1007T belonged to the genus Sphingomonas and was most closely related to Sphingomonas pruni IFO 15498T (97.3 %), Sphingomonas mali IFO 15500T (97.2 %), Sphingomonas japonica KC7T (97.2 %) and Sphingomonas koreensis JSS26T (97.0 %). This affiliation of strain 1007T to the genus Sphingomonas was confirmed by the presence of Q-10 as the major ubiquinone, sphingoglycolipid, C14 : 0 2-OH and by the absence of 3-hydroxy fatty acids. The major polyamine was homospermidine. The main cellular fatty acids included summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. Based on the low level of DNA–DNA relatedness (ranging from 26.1 % to 58.7 %) to these type strains of species of the genus Sphingomonas and unique phenotypic characteristics, strain 1007T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas yantingensis sp. nov. is proposed. The type strain is 1007T ( = DSM 27244T = JCM 19201T = CCTCC AB 2013146T).