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Pelolinea submarina gen. nov., sp. nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment

Imachi, Hiroyuki, Sakai, Sanae, Lipp, Julius S., Miyazaki, Masayuki, Saito, Yumi, Yamanaka, Yuko, Hinrichs, Kai-Uwe, Inagaki, Fumio, Takai, Ken
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 3 pp. 812-818
DNA, bacteria, carbohydrates, methanogens, monounsaturated fatty acids, new genus, nucleotide sequences, pH, ribosomal RNA, sediments, sequence analysis, sodium chloride, sphingolipids, yeast extract, Japan
A novel, anaerobic filamentous bacterium, strain MO-CFX1T, was isolated from a methanogenic community, which was originally established from subseafloor sediments collected from off the Shimokita Peninsula, Japan. Cells were non-spore-forming, non-motile, Gram-stain-negative and filamentous. The filaments were longer than 10 µm and 130–150 nm in width. Growth of the strain was observed at 10–37 °C (optimum 25–30 °C), at pH 5.5–8.5 (optimum pH 7.0) and in 0–50 g NaCl l−1 (optimum 15 g NaCl l−1). The strain was able to grow with a number of carbohydrates in the presence of yeast extract. The major cellular fatty acids were monounsaturated C18 : 1ω9, C16 : 1ω7 and saturated C18 : 0 and C16 : 0. The intact polar lipids of the strain were dominated by diacylglyceride and sphingolipid core lipid structures with monoglycosidic, mixed phosphomonoglycosidic and fatty-acid-modified monoglycosidic polar head groups. The G+C content of the genomic DNA was 52.4 mol%. Based on the comparative 16S rRNA gene sequence analysis, strain MO-CFX1T was affiliated with the class Anaerolineae within the phylum Chloroflexi and was most closely related to Leptolinea tardivitalis YMTK-2T (sequence identity of 91.0 %). Based on phenotypic and genetic properties of the novel isolate, we propose a novel species representing a new genus Pelolinea submarina gen. nov., sp. nov., for strain MO-CFX1T ( = JCM 17238T, = KCTC 5975T). This is the first formal description, to our knowledge, of an isolate of the phylum Chloroflexi from the deep-sea sedimentary environment.