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Lysobacter panacisoli sp. nov., isolated from ginseng soil

Author:
Choi, Jung-Hye, Seok, Ji-Hye, Cha, Ju-Hee, Cha, Chang-Jun
Source:
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 7 pp. 2193-2197
ISSN:
1466-5034
Subject:
DNA, Lysobacter, Panax, fatty acids, microbiology, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, sequence analysis, soil, ubiquinones, South Korea
Abstract:
A novel bacterial strain, designated CJ29T, was isolated from ginseng soil of Anseong in South Korea. Cells of strain CJ29T were Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile. Strain CJ29T grew optimally at 28–30 °C and pH 7.0. Based on 16S rRNA gene sequence analysis, strain CJ29T was shown to belong to the genus Lysobacter within the class Gammaproteobacteria and was related most closely to Lysobacter soli DCY21T (98.5 % similarity) and Lysobacter niastensis GH41-7T (98.2 %). DNA–DNA relatedness between strain CJ29T and its closest relatives was below 55.6 %. The predominant cellular fatty acids of strain CJ29T were iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1ω9c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major isoprenoid quinone was ubiquinone 8 (Q-8). The G+C content of the genomic DNA was 65.6 mol%. Phenotypic, genotypic and phylogenetic characteristics strongly supported the differentiation of strain CJ29T from related species of the genus Lysobacter. On the basis of data from this polyphasic taxonomic study, strain CJ29T is considered to represent a novel species of the genus Lysobacter, for which the name Lysobacter panacisoli sp. nov. is proposed. The type strain is CJ29T ( = KACC 17502T = JCM 19212T).
Agid:
1182119