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Lysinibacillus halotolerans sp. nov., isolated from saline-alkaline soil

Kong, Delong, Wang, Yanwei, Zhao, Bingqiang, Li, Yanting, Song, Jinlong, Zhai, Yi, Zhang, Chi, Wang, Huimin, Chen, Xiaorong, Zhao, Bin, Ruan, Zhiyong
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 8 pp. 2593-2598
DNA, Lysinibacillus, bacteria, chemotaxonomy, fatty acids, glycolipids, menaquinones, nucleic acid hybridization, nucleotide sequences, pH, peptidoglycans, phosphatidylethanolamines, phylogeny, ribosomal RNA, salt tolerance, sodium chloride, soil, soil sampling, temperature, China
A novel aerobic, halotolerant bacterium, designated strain LAM612T, was isolated from saline-alkaline soil samples from Lingxian County, Shandong Province, China. Cells of strain LAM612T were Gram-reaction-positive, endospore-forming, motile and rod-shaped. The optimal temperature and pH for growth were 35 °C and pH 6.0, respectively. Strain LAM612T could grow in the presence of up to 10 % (w/v) NaCl. The genomic DNA G+C conten was 36.4 mol% as detected by the Tm method. Comparative analysis of 16S rRNA gene sequences revealed that LAM612T was closely related to Lysinibacillus sinduriensis KACC 16611T (98.0 %), L. chungkukjangi KACC 16626T (97.5 %), L. massiliensis KCTC 13178T (97.4 %), L. xylanilyticus KACC 15113T (97.2 %), L. macroides DSM 54T (97.0 %) and L. manganicus DSM 26584T (96.5 %). The DNA–DNA hybridization values between strain LAM612T and its closest relatives ranged from 20.6 % to 41.9 %. The major fatty acids of strain LAM612T were iso-C15 : 0 (40.8 %), iso-C16 : 0 (15.2 %) and anteiso-C15 : 0 (10.8 %). The cell-wall peptidoglycan content was A4α (l-Lys–d-Asp). The predominant menaquinone was MK-7 and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, five unknown glycolipids and an unknown lipid. Based on the DNA–DNA hybridization results and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM612T could be distinguished from the recognized species of the genus Lysinibacillus, and was suggested to represent a novel species of this genus, for which the name Lysinibacillus halotolerans sp. nov. is proposed. The type strain is LAM612T ( = ACCC 00718T = JCM 19611T).