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Chitinimonas prasina sp. nov., isolated from lake water

Li, Yi, Zhu, Hong, Lai, Qiliang, Lei, Xueqian, Chen, Zhangran, Zhang, Huajun, Tian, Yun, Zheng, Wei, Zheng, Tianling
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 9 pp. 3005-3009
Burkholderiaceae, DNA, fatty acid composition, fatty acids, genes, lakes, microbiology, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, physiology, ribosomal RNA, sequence homology, sodium chloride, China
A Gram-stain-negative, elongated rod-shaped, motile by gliding, green-pigmented, aerobic bacterial strain, designated LY03T, was isolated from lake water in Xiamen, Fujian Province, China. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Chitinimonas, which belongs to the family Burkholderiaceae. Strain LY03T was most closely related to Chitinimonas taiwanensis LMG 22011T (96.02 % 16S rRNA gene sequence similarity), followed by Chitinimonas koreensis KACC 11467T (94.85 %), and the three strains formed a distinct lineage from other strains in the phylogenetic analyses. Optimum conditions for growth were 37 °C, pH 7–9 and without NaCl. The major fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and C10 : 0 3-OH. The DNA G+C content of strain LY03T was 63.6 mol% and the major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown polar lipids and unidentified phospholipids. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LY03T from all other members of the genus Chitinimonas. On the basis of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain LY03T represents a novel species of the genus Chitinimonas, for which the name Chitinimonas prasina sp. nov. is proposed. The type strain is LY03T ( = MCCC 1F01209T = KCTC 32574T).