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Rhizobium populi sp. nov., an endophytic bacterium isolated from Populus euphratica

Author:
Rozahon, Manziram, Ismayil, Nurimangul, Hamood, Buayshem, Erkin, Raziya, Abdurahman, Mehfuzem, Mamtimin, Hormathan, Abdukerim, Muhtar, Lal, Rup, Rahman, Erkin
Source:
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 9 pp. 3215-3221
ISSN:
1466-5034
Subject:
DNA, Populus euphratica, Rhizobium, bacteria, chemotaxonomy, endophytes, fatty acids, genes, nucleotide sequences, pH, phosphatidylcholines, phosphatidylethanolamines, phylogeny, ribosomal RNA, rivers, sequence homology, sodium chloride, stems, temperature, trees, China
Abstract:
An endophytic bacterium, designated K-38T, was isolated from the storage liquid in the stems of Populus euphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain K-38T was found to be rod-shaped, Gram-stain-negative, aerobic, non-motile and non-spore-forming. Strain K-38T grew at temperatures of 25–37 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, pH 7.5) and in the presence of 0–3 % (w/v) NaCl with 1 % as the optimum concentration for growth. According to phylogenetic analysis based on 16S rRNA gene sequences, strain K-38T was assigned to the genus Rhizobium with highest 16S rRNA gene sequence similarity of 97.2 % to Rhizobium rosettiformans W3T, followed by Rhizobium nepotum 39/7T (96.5 %) and Rhizobium borbori DN316T (96.2 %). Phylogenetic analysis of strain K-38T based on the protein coding genes recA, atpD and nifH confirmed (similarities were less than 90 %) it to be a representative of a distinctly delineated species of the genus Rhizobium. The DNA G+C content was determined to be 63.5 mol%. DNA–DNA relatedness between K-38T and R. rosettiformans W3T was 48.4 %, indicating genetic separation of strain K-38T from the latter strain. The major components of the cellular fatty acids in strain K-38T were revealed to be summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c; 57.2 %), C16 : 0 (13.6 %) and summed feature 2 (comprising C12 : 0 aldehyde, C14 : 0 3-OH/iso-C16 : 1 I and/or unknown ECL 10.928; 11.0 %). Polar lipids of strain K-38T include phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids and two unidentified phospholipids. Q-10 was the major quinone in strain K-38T. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain K-38T represents a novel species of the genus Rhizobium, for which the name Rhizobium populi sp. nov. is proposed. The type strain is K-38T ( = CCTCC AB 2013068T = NRRL B-59990T = JCM 19159T).
Agid:
1182274