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Burkholderia jiangsuensis sp. nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil

Author:
Liu, Xu-Yun, Li, Chun-Xiu, Luo, Xiao-Jing, Lai, Qi-Liang, Xu, Jian-He
Source:
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 9 pp. 3247-3253
ISSN:
1466-5034
Subject:
Burkholderia glathei, bacteria, ethanolamine, fatty acids, gelatin, genes, glucose, glycerol, nitrates, nitrites, nucleotide sequences, parathion-methyl, phospholipids, phylogeny, polluted soils, ribosomal RNA, sequence homology, temperature, ubiquinones, wastelands, China
Abstract:
A methyl parathion (MP) degrading bacterial strain, designated MP-1T, was isolated from a waste land where pesticides were formerly manufactured in Jiangsu province, China. Polyphasic taxonomic studies showed that MP-1T is a Gram-stain-negative, non-spore-forming, rod-shaped and motile bacterium. The bacterium could grow at salinities of 0–1 % (w/v) and temperatures of 15–40 °C. Strain MP-1T could reduce nitrate to nitrite, utilize d-glucose and l-arabinose, but not produce indole, or hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that MP-1T belongs to the genus Burkholderia, showing highest sequence similarity to Burkholderia grimmiae DSM 25160T (98.5 %), and similar strains including Burkholderia zhejiangensis OP-1T (98.2 %), Burkholderia choica LMG 22940T (97.5 %), Burkholderia glathei DSM 50014T (97.4 %), Burkholderia terrestris LMG 22937T (97.2 %) and Burkholderia telluris LMG 22936T (97.0 %). In addition, the gyrB and recA gene segments of strain MP-1T exhibited less than 89.0 % and 95.1 % similarities with the most highly-related type strains indicated above. The G+C content of strain MP-1T was 62.6 mol%. The major isoprenoid quinone was ubiquinone Q-8. The predominant polar lipids comprised phosphatidyl ethanolamine, phosphatidyl glycerol, aminolipid and phospholipid. The principal fatty acids in strain MP-1T were C18 : 1ω7c/C18 : 1ω6c (23.3 %), C16 : 0 (16.8 %), cyclo-C17 : 0 (15.0 %), C16 : 1ω7c/C16 : 1ω6 (8.5 %), cyclo-C19 : 0ω8c (8.1 %), C16 : 1 iso I/C14 : 0 3-OH (5.7 %), C16 : 0 3-OH (5.6 %) and C16 : 02-OH (5.1 %). The DNA–DNA relatedness values between strain MP-1T and the three type strains (B. grimmiae DSM 25160T, B. zhejiangensis OP-1T and B. glathei DSM 50014T) ranged from 24.6 % to 37.4 %. In accordance with phenotypic and genotypic characteristics, strain MP-1T represents a novel species of the genus Burkholderia, for which the name Burkholderia jiangsuensis sp. nov. is proposed, the type strain is MP-1T (LMG 27927T = MCCC 1K00250T).
Agid:
1182279