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Global Analysis of the Burkholderia thailandensis Quorum Sensing-Controlled Regulon

Majerczyk, Charlotte, Brittnacher, Mitchell, Jacobs, Michael, Armour, Christopher D., Radey, Mathew, Schneider, Emily, Phattarasokul, Somsak, Bunt, Richard, Greenberg, E. Peter
Journal of bacteriology 2014 v.196 no.7 pp. 1412-1424
Burkholderia mallei, Burkholderia pseudomallei, Burkholderia thailandensis, bacteriology, exopolysaccharides, mutants, quorum sensing, regulon, saprophytes, secondary metabolites, transcriptomics
Burkholderia thailandensis contains three acyl-homoserine lactone quorum sensing circuits and has two additional LuxR homologs. To identify B. thailandensis quorum sensing-controlled genes, we carried out transcriptome sequencing (RNA-seq) analyses of quorum sensing mutants and their parent. The analyses were grounded in the fact that we identified genes coding for factors shown previously to be regulated by quorum sensing among a larger set of quorum-controlled genes. We also found that genes coding for contact-dependent inhibition were induced by quorum sensing and confirmed that specific quorum sensing mutants had a contact-dependent inhibition defect. Additional quorum-controlled genes included those for the production of numerous secondary metabolites, an uncharacterized exopolysaccharide, and a predicted chitin-binding protein. This study provides insights into the roles of the three quorum sensing circuits in the saprophytic lifestyle of B. thailandensis, and it provides a foundation on which to build an understanding of the roles of quorum sensing in the biology of B. thailandensis and the closely related pathogenic Burkholderia pseudomallei and Burkholderia mallei.