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Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance

Author:
Wang, Zhi Wei, Wang, Chuan, Gao, Lei, Mei, Shi Yong, Zhou, Yuan, Xiang, Chang Ping, Wang, Ting
Source:
Journal of experimental botany 2013 v.64 no.7 pp. 2041-2048
ISSN:
0022-0957
Subject:
DNA-directed RNA polymerase, Raphanus sativus, alleles, crops, cytoplasmic male sterility, heterosis, heterozygosity, homozygosity, hybridization, molecular cloning, overdominance, proteins, radishes
Abstract:
The practice of hybridization has greatly contributed to the increase in crop productivity. A major component that exploits heterosis in crops is the cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system. Through positional cloning, it is shown that heterozygous alleles (RsRf3-1/RsRf3-2) encoding pentatricopeptide repeat (PPR) proteins are responsible for restoring fertility to cytoplasmic male-sterile radish (Raphanus sativus L.). Furthermore, it was found that heterozygous alleles (RsRf3-1/RsRf3-2) show higher expression and RNA polymerase II occupancy in the CMS cytoplasmic background compared with their homozygous alleles (RsRf3-1/RsRf3-1 or RsRf3-2/RsRf3-2). These data provide new insights into the molecular mechanism of fertility restoration to cytoplasmic male-sterile plants and illustrate a case of overdominance.
Agid:
1189082