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The phosphoproteome in regenerating protoplasts from Physcomitrella patens protonemata shows changes paralleling postembryonic development in higher plants

Author:
Wang, Xiaoqin, Qi, Meiyan, Li, Jingyun, Ji, Zhongzhong, Hu, Yong, Bao, Fang, Mahalingam, Ramamurthy, He, Yikun
Source:
Journal of experimental botany 2014 v.65 no.8 pp. 2093-2106
ISSN:
0022-0957
Subject:
Physcomitrella patens, organogenesis, proteins, protonemata, protoplasts
Abstract:
The moss Physcomitrella patens is an ideal model plant to study plant developmental processes. To better understand the mechanism of protoplast regeneration, a phosphoproteome analysis was performed. Protoplasts were prepared from protonemata. By 4 d of protoplast regeneration, the first cell divisions had ensued. Through a highly selective titanium dioxide (TiO₂)-based phosphopeptide enrichment method and mass spectrometric technology, more than 300 phosphoproteins were identified as protoplast regeneration responsive. Of these, 108 phosphoproteins were present on day 4 but not in fresh protoplasts or those cultured for 2 d. These proteins are catalogued here. They were involved in cell-wall metabolism, transcription, signal transduction, cell growth/division, and cell structure. These protein functions are related to cell morphogenesis, organogenesis, and development adjustment. This study presents a comprehensive analysis of phosphoproteome involved in protoplast regeneration and indicates that the mechanism of plant protoplast regeneration is similar to that of postembryonic development.
Agid:
1189568