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Development of a genetic linkage map in hazelnut (corylus avellana) for the detection of QTLS

Beltramo, C., Boccacci, P., Sandoval Prando, M. A., Botta, R., Portis, E.
Acta horticulturae 2014 no.1052 pp. 99-104
Corylus avellana, Curculionidae, agronomic traits, amplified fragment length polymorphism, chromosome mapping, cultivars, flowering, genetic markers, hazelnuts, loci, marker-assisted selection, microsatellite repeats, mites, parents, piedmont, progeny, quantitative trait loci, ripening, single nucleotide polymorphism, suckering, trees, vigor
‘Tonda Gentile delle Langhe’ (TGdL) is the dominant hazelnut cultivar in Piedmont due to the unquestioned technological and quality characteristics of its nuts. The improvement of TGdL by breeding is one of the objectives of the projects of the University of Torino. A F1 progeny (295 plants) of ‘Tonda Gentile delle Langhe’ × ‘Hall’s Giant’ was obtained in 2009 to study the segregation of traits of agronomic and technological interest and develop marker assisted selection. Parents differ for big bud mite and nut weevil tolerance, tree vigour, time of budburst, flowering and ripening time, and nut characteristics. A first set of 142 plants of the progeny was analyzed at 92 SSR loci. In 2011, tree vigour, suckering habit, and time of budburst were observed in 295 plants. The preliminary data showed a large variability of the traits with a normal distribution for vigour and time of budburst. Molecular analysis and field observations were statistically analyzed to obtain a first linkage map consisting of 10 LGs covering 452 cM. In the next years, the map will be saturated using further molecular markers, including AFLP and SNPs; more field and lab data will be collected for getting reliable results and detecting the QTL regions in the map.