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Nigella sativa Oil Reduces Aluminium Chloride-Induced Oxidative Injury in Liver and Erythrocytes of Rats

Author:
Bouasla, Ihcene, Bouasla, Asma, Boumendjel, Amel, Messarah, Mahfoud, Abdennour, Cherif, Boulakoud, Mohamed Salah, El Feki, Abdelfattah
Source:
Biological trace element research 2014 v.162 no.1-3 pp. 252-261
ISSN:
0163-4984
Subject:
Nigella sativa, alanine transaminase, albumins, alkaline phosphatase, aluminum, aluminum chloride, antioxidant activity, aspartate transaminase, bilirubin, body weight, catalase, diet, erythrocytes, flavonoids, glutathione, glutathione peroxidase, hemoglobin, lactate dehydrogenase, leukocyte count, liver, malondialdehyde, oils, oxidative stress, protective effect, rats, superoxide dismutase, tannins, toxicity
Abstract:
The present study was planned to investigate the protective effects of Nigella sativa oil (NSO) supplementation against aluminium chloride (AlCl₃)-induced oxidative damage in liver and erythrocytes of rats. Simultaneously, a preliminary phytochemical study was affected in order to characterize the bioactive components containing in the NSO using chemical assays. The antioxidant capacities of NSO were evaluated by DPPH assay. The results showed that NSO was found to contain large amounts of total phenolics, flavonoids and tannins. Twenty-four rats were equally divided into two groups, in which group A received standard diet, whereas group B treated daily with an oral gavage dose of 2 ml NSO/kg body weight. After 5 weeks pretreatment, both groups were divided again into two subgroups (A and B) of six animals each and treated for other 3 weeks. Therefore, subgroup A1 was served as a control which received standard diet, but subgroup A2 received AlCl₃(34 mg/kg bw mixed with food). Subgroup B1 received both AlCl₃and NSO; however, subgroup B2 received NSO only. Results showed that AlCl₃exhibited an increase in white blood cell counts and a marked decrease in erythrocyte counts and haemoglobin content. Plasma aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate dehydrogenase activities and total bilirubin concentration were higher in AlCl₃group than those of the control, while albumin and total protein concentration were significantly lower. Compared to the control, a significant raise of hepatic and erythrocyte malondialdehyde level associated with a decrease in reduced glutathione content, glutathione peroxidase, superoxide dismutase and catalase, activities of AlCl₃treated rats. However, the administration of NSO alone or combined with AlCl₃has improved the status of all parameters studied. It can be concluded that AlCl₃has induced the oxidative stress, altered the biochemical parameters and the hepatic histological profile, but the supplementation of NSO has alleviated such toxicity.
Agid:
1192550