Jump to Main Content
A computational investigation of kinetoplastid trans-splicing
- Gopal, Shuba, Awadalla, Saria, Gaasterland, Terry, Cross, George AM
- Genome biology 2005 v.6 no.11 pp. 1130
- Leishmania major, chromosomes, genes, messenger RNA, prediction, trans-splicing
- Trans-splicing is an unusual process in which two separate RNA strands are spliced together to yield a mature mRNA. We present a novel computational approach which has an overall accuracy of 82% and can predict 92% of known trans-splicing sites. We have applied our method to chromosomes 1 and 3 of Leishmania major, with high-confidence predictions for 85% and 88% of annotated genes respectively. We suggest some extensions of our method to other systems.