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Application of two-dimensional chromatography to the characterization of macromolecules and biomacromolecules
- Kilz, Peter, Radke, Wolfgang
- Analytical and bioanalytical chemistry 2015 v.407 no.1 pp. 193-215
- gel chromatography, molecular weight, polymers
- Modern polymeric materials are heterogeneous with respect to different structural features, for instance molar mass, composition, and architecture. One-dimensional separation methods such as size-exclusion chromatography (SEC) are insufficient to fully resolve the multidimensional distributions of such complex materials. Therefore, two-dimensional separation methods are increasingly employed to characterize macromolecules. The present article describes in detail the advantages and experimental aspects of two-dimensional macromolecular separations. Selected examples will be discussed to explain the strategies used to separate macromolecules with respect to specific structural features.