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Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil

Author:
Bouras, Noureddine, Meklat, Atika, Zitouni, Abdelghani, Mathieu, Florence, Schumann, Peter, Spröer, Cathrin, Sabaou, Nasserdine, Klenk, Hans-Peter
Source:
Antonie van Leeuwenhoek 2015 v.107 no.2 pp. 313-320
ISSN:
0003-6072
Subject:
Nocardiopsis alba, Nocardiopsis lucentensis, chemotaxonomy, fatty acids, menaquinones, mycelium, new species, nucleotide sequences, pH, peptidoglycans, phosphatidylcholines, phosphatidylethanolamines, ribosomal RNA, sequence analysis, sodium chloride, soil, spores, temperature, Algeria
Abstract:
An alkalitolerant actinomycete strain, designated B32ᵀ, was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0–5 % (w/v) and the optimum growth temperature and pH were found to be 25–35 °C and 7.0–10.0 °C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32ᵀ were identified as MK-10 (H₄) and MK-11 (H₄). The major fatty acids were found to be iso-C₁₆:₀ and anteiso-C₁₅:₀. The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32ᵀ are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32ᵀ is most closely related to Nocardiopsis alba DSM 43377ᵀ (98.7 %), Nocardiopsis lucentensis DSM 44048ᵀ (98.6 %), Nocardiopsis aegyptia DSM 44442ᵀ (98.6 %), Nocardiopsis sinuspersici HM6ᵀ (98.6 %) and Nocardiopsis arvandica HM7ᵀ (98.5 %). However, the DNA–DNA relatedness values between strain B32ᵀ and the closely related type strains were 17.9, 14.6, 31.1, 27.1 and 14.1 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain B32ᵀ should be classified as representative of a novel species, for which the name Nocardiopsis algeriensis sp. nov. is proposed. The type strain is B32ᵀ (=DSM 45462ᵀ = CECT 8712ᵀ).
Agid:
1200030