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Efficacy of Aspergillus sp. for Degradation of Chlorpyrifos in Batch and Continuous Aerated Packed Bed Bioreactors

Author:
Yadav, Maya, Srivastva, Navnita, Shukla, Awadhesh Kumar, Singh, Ram Sharan, Upadhyay, Siddh Nath, Dubey, Suresh Kumar
Source:
Applied biochemistry and biotechnology 2015 v.175 no.1 pp. 16-24
ISSN:
0273-2289
Subject:
Aspergillus, biochemistry, biodegradation, bioreactors, chlorpyrifos, foams, inoculum, pH, polyurethanes, temperature
Abstract:
Aerobic biodegradation of chlorpyrifos (CP) by Aspergillus sp. was investigated in batch and continuous packed bed bioreactors. The optimal process parameters for achieving the maximum removal efficiency (RE), determined using a batch bioreactor packed with polyurethane foam pieces, were inoculum level: 2.5 mg (wet weight) mL⁻¹, pH 7.0, temperature 28 °C, DO 5.8 mg L⁻¹, and CP concentration 300 mg L⁻¹. The continuous packed bed bioreactor was operated at flow rates ranging from 10 to 40 mL h⁻¹while keeping other parameters at their optimal level. Steady-state CP removal efficiencies greater than 85 % were obtained up to the inlet loading of 180 mg L⁻¹ d⁻¹. The continuous bioreactor behaved as a plug flow unit and was able to stabilize quickly after perturbation in the inlet loading.
Agid:
1200495